Res. Plant Dis > Volume 25(3); 2019 > Article
Research in Plant Disease 2019;25(3):131-135.
DOI:    Published online September 30, 2019.
Diagnosis of Cylindrocarpon destructans Using Enzyme-Linked Immunosorbent Assay
Taiying Li1, Sungyeon Ji1, Boknam Jung2, Bo Yeon Kim1, Kwang Sik Lee3, Mun Won Seo3, Sung Woo Lee3, Jungkwan Lee1, Seung-Ho Lee3
1Department of Applied Biology, Dong-A University, Busan 49315, Korea
2Forest Insect Pests and Diseases Division, National Institute of Forest Science, Seoul 02455, Korea
3Ginseng Research Division, National Institute of Horticultural and Herbal Science, Eumseong 27715, Korea
Correspondence:  J. Lee, S.-H. Lee,*J. Lee
Tel: +82-51-200-7554
Fax: +82-51-200-7505
*S.-H. Lee
Tel: +82-43-871-5609
Received: July 11, 2019   Accepted: August 30, 2019
Cylindrocarpon destructans causes ginseng root rot and produces radicicol that has an antifungal effect. In this study, we developed a method to detect this fungus using enzyme-linked immunosorbent assay (ELISA). Secreted proteins of C. destructans were used as antigens to obtain C. destructans-specific IgG from mouse. Out of 318 monoclonal antibodies generated from mouse, two antibodies (Cd7-2-2 and Cd7-2-10) showed highest specificity and sensitivity. Indirect ELISA using both antigens successfully detected C. destructans in soils, but direct ELISA using IgG conjugated with horseradish peroxidase failed to detect antigens in soils. The indirect ELISA developed here can efficiently detect the fungus and help manage ginseng root rot disease in fields.
Key Words: Cylindrocarpon destructans, Diagnosis, Enzyme-linked immunosorbent assay, Fusarium solani, Ginseng root rot

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