Diagnosis of <i>Cylindrocarpon destructans</i> Using Enzyme-Linked Immunosorbent Assay

Li, Taiying; Ji, Sungyeon; Jung, Boknam; Kim, Bo Yeon; Lee, Kwang Sik; Seo, Mun Won; Lee, Sung Woo; Lee, Jungkwan; Lee, Seung-Ho

doi:2019.25.3.131

Res. Plant Dis > Volume 25(3); 2019 > Article

Research in Plant Disease 2019;25(3):131-135.
DOI: https://doi.org/10.5423/RPD.2019.25.3.131 Published online September 30, 2019.

Diagnosis of Cylindrocarpon destructans Using Enzyme-Linked Immunosorbent Assay

Taiying Li¹, Sungyeon Ji¹, Boknam Jung², Bo Yeon Kim¹, Kwang Sik Lee³, Mun Won Seo³, Sung Woo Lee³, Jungkwan Lee¹, Seung-Ho Lee³

¹Department of Applied Biology, Dong-A University, Busan 49315, Korea
²Forest Insect Pests and Diseases Division, National Institute of Forest Science, Seoul 02455, Korea
³Ginseng Research Division, National Institute of Horticultural and Herbal Science, Eumseong 27715, Korea

Correspondence: J. Lee, S.-H. Lee,*J. Lee
Tel: +82-51-200-7554
Fax: +82-51-200-7505
E-mail: jungle@dau.ac.kr
*S.-H. Lee
Tel: +82-43-871-5609
E-mail: lsh750409@korea.kr

Received: July 11, 2019 Accepted: August 30, 2019

Abstract

Cylindrocarpon destructans causes ginseng root rot and produces radicicol that has an antifungal effect. In this study, we developed a method to detect this fungus using enzyme-linked immunosorbent assay (ELISA). Secreted proteins of C. destructans were used as antigens to obtain C. destructans-specific IgG from mouse. Out of 318 monoclonal antibodies generated from mouse, two antibodies (Cd7-2-2 and Cd7-2-10) showed highest specificity and sensitivity. Indirect ELISA using both antigens successfully detected C. destructans in soils, but direct ELISA using IgG conjugated with horseradish peroxidase failed to detect antigens in soils. The indirect ELISA developed here can efficiently detect the fungus and help manage ginseng root rot disease in fields.

Key Words: Cylindrocarpon destructans, Diagnosis, Enzyme-linked immunosorbent assay, Fusarium solani, Ginseng root rot