Research in Plant Disease 2014;20(1):21-24.
Published online December 15, 2014.
배추 뿌리혹병균 Plasmodiophora brassicae의 종 특이적 프라이머 개발
최진수 · 양슬기 · 송정영 · 김홍기*
충남대학교 응용생물학과
 
Development of Species-Specific Primers for Plasmodiophora brassicae, Clubroot Pathogen of Kimchi Cabbage
Jin Su Choi, Seul Gi Yang, Jeong Young Song and Hong Gi Kim*
Department of Applied Biology, Chungnam National University, Daejeon 305-764, Korea
Correspondence:  <p>Hong Gi Kim</p><p>Tel : +82-4,

Hong Gi Kim

Tel : +82-42-821-5768
Fax: +82-42-823-8679
E-mail: hgkim@cnu.ac.kr

Received: November 29, 2012   Revised: February 14, 2014   Accepted: February 21, 2014
Abstract
Clubroot caused by the obligate biotrophic protist Plasmodiophora brassicae Woronin is one of the most damaging diseases of Brassicaceae family. In this study, we developed species-specific primer sets for rapid and accurate detection of P. brassicae. The primer sets developed amplified a specific fragment only from P. brassicae DNA while they did not amplify a band from 10 other soilborne pathogens or from Kimchi cabbage. In sensitivity test, the species-specific primer set ITS1-1/ITS1-2 could work for approximately 10 spores/ml of genomic DNA showing more sensitivity and accuracy than previous methods. With quantitative real-time PCR test, the primer set detected less spores of P. brassicae than before, confirming that the species-specific primer set could be useful for rapid and accurate detection of P. brassicae.
Key Words: Clubroot pathogen, Detection, Plasmodiophora brassicae, Quantitative real-time PCR, Speciesspecific primer


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