Research in Plant Disease 2011;17(2):161-168.
Published online August 30, 2011.
효율적인 무 뿌리혹병 저항성 검정법 확립
조수정, 장경수, 최용호, 김진철, 최경자
Development of Convenient Screening Method for Resistant Radish to Plasmodiophora brassicae
Su Jung Jo, Kyoung Soo Jang, Yong Ho Choi, Jin Cheol Kim, Gyung Ja Choi
To establish simple and reliable screening method for resistant radish to Plasmodiophora brassicae Woron. using soil-drenching inoculation, the development of clubroot on radish seedlings inoculated with P. brassicae GN-1 isolate according to several conditions such as inoculum concentration, plant growth stage and incubation period after inoculation was studied. To select resistant radish against clubroot, 10-day-old seedlings were inoculated with P. brassicae by drenching the roots with the spore suspension of the pathogen to give 1×10(9) spores/pot. The inoculated seedlings were incubated in a growth chamber at 20℃ for 3 days then cultivated in a greenhouse (20±5℃) for 6 weeks. Under the optimum conditions, 46 commercial cultivars of radish were tested for resistance to YC-1 (infecting 15 clubroot-resistant cultivars of Chinese cabbage) and GN-1 (wild type) isolates of P. brassicae. Among them, thirty-five cultivars showed resistance to both isolates and one cultivar represented susceptible response to the pathogens. On the other hand, the other cultivars showed different responses against the tested P. brassicae pathogens. The results suggest that this method is an efficient system for screening radish with resistance to clubroot.
Key Words: Breeding, Clubroot, Crucifer crops, Cultivar, Resistance

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