Research in Plant Disease 2010;16(3):219-223.
Published online December 30, 2010.
참나무류에 시들음병을 일으키는 Raffaelea quercus-mongolicae와 R. quercivora의 유전적 특성
서상태, 김경희, 이상현, 권용남, 신창훈, 김혜정, 이상용
 
Genotypic Characterization of Oak Wilt Pathogen Raffaelea quercus-mongolicae and R. quercivora Strains
Sang Tae Seo, Kyung Hee Kim, Sang Hyun Lee, Yong Nam Kwon, Chang Hoon Shin, Hye Jeong Kim, Sang Yong Lee
Abstract
Recently, the oak wilt diseases especially on Quercus mongolica, have been increasing in various districts of Korea. A collection of 38 strains of the oak wilt pathogen Raffaelea qeurcus-mongolicae and R. quecivora isolated from Quercus spp. in Korea and Japan was characterized by β-tubulin gene sequence and randomly amplified polymorphic DNA (RAPD) analysis. In cluster analysis based on β-tubulin gene sequence the strains were divided into 4 clusters, of which clusters 2 and 4 were composed of Japanese strains except for one Korean strain. RAPD analysis showed that they were also effectively differentiated by a strong RAPD fragments. On the basis of the two genetic analysis, significant differences were detected between Korean strains and Japanese strains.
Key Words: Raffaelea qeurcus-mongolicae, R. quecivora, RAPD, β-tubulin


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