Research in Plant Disease 2005;11(2):140-145.
Published online December 30, 2005.
제주지역에서 분리한 감자 줄기검은병균의 유전적 특성
서상태, 이승돈, 이중섭, 한경숙, 장한익, 임춘근
Genetic Characterization of Potato Blackleg Strains from Jeju Island
Sang Tae Seo, Seung Don Lee, Jung Sup Lee, Kyoung Suk Han, Han Ik Jang, Chun Keun Lim
A collection of 12 Erwinia carotovora strains from blackleg diseased potato in Jeju island was characterized genetic diversity by E. carotovora subsp. atroseptica (Eca)-specific PCR, PCR-RFLP of the two genes (16SrRNA and pel) and repetitive sequence PCR (ERIC-PCR). The results were compared with those of the other E. carotovora representative strains. None of the blackleg strains produced PCR amplicons with Eca-specific primers in contrast to the single 690 bp amplicon obtained with Eca strains. In addition, on the basis of pel gene RFLP with Sau3AI, the blackleg strains belonged to the pattern 2 whereas Eca strains belonged to the other one (pattern 3). By analysis of 16S rDNA RFLP generated with Hinf I, the most strains including the E. carotovora subsp. carotovora (Ecc) representative strains used in this study belonged to the pattern 1 whereas the blackleg strains belonged to the pattern 2 except for one strain. Moreover, ERIC-PCR analysis showed that the blackleg strains were closely related to each other and had an unique DNA band. Based on these molecular approaches, we have confirmed that the blackleg disease of potato is caused by a different E. carotovora from Eca and Ecc in Jeju island.
Key Words: Blackleg, Erwinia carotovora, PCR, RFLP, Soft rot

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