Genetic Diversity of Ralstonia solanacearum Strains Isolated from Pepper and Tomato Plants in Korea

Seo, Sang Tae; Park, Jong Han; Han, Kyoung Suk; Cheong, Seung Ryong; Lee, Seung Don

Research in Plant Disease 2007;13(1):24-29.
Published online April 30, 2007.

우리나라에 분포하는 고추와 토마토 풋마름병균(Ralstonia solanacearum) 계통들의 유전적 다양성

서상태, 박종한, 한경숙, 정승룡, 이승돈

Genetic Diversity of Ralstonia solanacearum Strains Isolated from Pepper and Tomato Plants in Korea

Sang Tae Seo, Jong Han Park, Kyoung Suk Han, Seung Ryong Cheong, Seung Don Lee

Abstract

A total of 35 strains of Ralstonia solanacearum isolated from wilted pepper and tomato plants in Korea were analyzed for their genetic diversity by bacteriological, pathological and molecular biological approaches. All the strains were identified as R. solanacearum biovar 4 on the basis of physiological and biochemical tests, and species-specific PCR primers. Pathogenicity of the strains was confirmed by inoculating on 4-week-old pepper and tomato seedlings. Using cluster analysis based on repetitive sequence-based polymerase chain reaction (rep-PCR) genomic fingerprints, R. solanacearum strains isolated from pepper and tomato in Korea divided into 6 groups showing a high degree of genetic diversity at 55% similarity level. The genetic diversity of strains was not significantly correlated with their geographic origins and host plants.

Key Words: Genetic diversity, Pepper, Ralstonia solanacearum, Tomato