Research in Plant Disease 2013;19(4):265-272.
Published online December 30, 2013.
고추에서 분리된 Ralstonia solanacearum 계통의 생리, 생화학 및 유전적 특성
이영기 ( Young Kee Lee ) , 강희완 ( Hee Wan Kang )
 
Physiological, Biochemical and Genetic Characteristics of Ralstonia solanacearum Strains Isolated from Pepper Plants in Korea
Young Kee Lee1 and Hee Wan Kang2,3*
1Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Korea
2Graduate School of Future Convergence Technology, Hankyong National University, Ansung 456-749, Korea
3Institute of Genetic Engineering, Hankyong National University, Ansung 456-749, Korea
Received: October 30, 2013   Revised: November 12, 2013   Accepted: November 19, 2013
Abstract
Totally sixty three bacteria were isolated from lower stems showing symptoms of bacterial wilt on pepper plants in 14 counties of 7 provinces, Korea. The isolates showed strong pathogenicity on red pepper (cv. Daewang) and tomato (cv. Seogwang) seedlings. All virulent bacteria were identified as Ralstonia solanacearum based on colony types, physiological and biochemical tests and polymerase chain reaction (PCR). All R. solanacearum isolates from peppers were race 1. The bacterial isolates consisted of biovar 3 (27%) and biovar 4 (73%). Based on polymorphic PCR bands generated by repetitive sequence (rep-PCR), the 63 R. solanacearum isolates were divided into 12 groups at 70% similarity level. These results will be used as basic materials for resistant breeding program and efficient control against bacterial wilt disease of pepper.
Key Words: Biovar, Classification, Pepper, Ralstonia solanacearum, Rep-PCR


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