Research in Plant Disease 2014;20(3):219-222.
Published online December 15, 2014.
Molecular Characterization of Tomato Yellow Leaf Curl Virus in Korea and the Construction of an Infectious Clone
Bong Choon Lee1*, Shigenori Ueda2, Young-Nam Yoon1, Dong Bum Shin1 and Hang-Won Kang1
1Crop Environment Research Division, National Institute of Crop Science, Rural Developement Administration, Suwon 441-857, Korea
2NARO Hokkaido Agricultural Research Center, Sapporo, Japan
Correspondence:  <p>Bong Choon Lee</p><p>Tel : +8,

Bong Choon Lee

Tel : +82-31-290-6791
Fax: +82-31-290-6773
E-mail: leebc21@korea.kr

Received: July 02, 2014   Revised: August 21, 2014   Accepted: August 22, 2014
Abstract

Several tomato production regions in Korea were surveyed for tomato yellow leaf curl disease (TYLCD). Tomato leaf samples showing TYLCD-like symptoms were collected from Tongyeong (To), Geoje (Gi), and Gimhae (Gh) cities of the southern part of Korea. Tomato yellow leaf curl virus (TYLCV) was detected and the full-length genomes of the isolates were sequenced. The TYLCV isolates found in Korea shared high sequence identity (> 99%) with TYLCV-IL [JR:Omu:Ng] (AB110217). Phylogenetic relationship analysis revealed that they formed two groups (with little genetic variability), and the To, Gj, and Gh isolates belonged to the TYLCV-IL group. An infectious clone of TYLCV-To (JQ013089) was constructed and agroinoculated into Nicotiana benthamiana, Nicotiana tabacum var. Xanthi, Petunia hybrida, Capsicum annuum, and Lycopersicon esculentum cv. Hausumomotaro. Agroinfection with a dimeric infectious clone of TYLCV-To induced severe leaf curling and stunting symptoms in these plants, excluding C. annuum. Tomato plants then developed typical yellow leaf curl symptoms.

Key Words: Agroinfection, Infectious clone, Sequence, Tomato yellow leaf curl virus


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