Development of RT-PCR and Nested PCR for Detecting Four Quarantine Plant Viruses Belonging to Nepovirus

Research in Plant Disease 2013;19(3):220-225.
Published online September 30, 2013.

RT-PCR과 nested PCR을 이용한 Nepovirus속 식물검역 바이러스 4종의 정밀진단

이시원 ( Si Won Lee ) , 강은하 ( Eun Ha Kang ) , 신용길 ( Yong Gil Shin ) , 이수헌 ( Su Heon Lee )

Development of RT-PCR and Nested PCR for Detecting Four Quarantine Plant Viruses Belonging to Nepovirus

Siwon Lee1,2, Eun-Ha Kang1,3, Yong-Gil Shin1* and Su-Heon Lee4,5**

¹Plant Quarantine Technology Center, Animal and Plant Qurantine Agency, Suwon 443-440, Korea
²Department of Microbiology, Dankook University, Cheonan 330-714, Korea
³Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Korea
⁴School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Korea
⁵Institute of Plant Medicine, Kyungpook National University, Daegu 702-701, Korea

Received: March 25, 2013 Revised: September 13, 2013 Accepted: September 17, 2013

Abstract

For quarantine purpose, we developed the RT- and nested PCR module of Tomato black ring virus (TBRV), Arabis mosaic virus (ArMV), Cherry leafroll virus (CLRV) and Grapevine fanleaf virus (GFLV). The PCR modules, developed in this study make diagnosis more convenient and speedy because of same PCR condition. And also, the methods are more accurate because it can check whether the result is contamination or not using the mutation-positive control. We discard or return the 27 cases of Nepovirus infection seed by employing the module past 3 years. This study provides a rapid and useful method for detection of four quarantine plant viruses.

Key Words: Detection, Nepovirus, Nested PCR, Quarantine, RT-PCR