An Easy Seedling Method to Screen Resistance of Powdery Mildew of Barley and Wheat

Park, Jong Chul; Park, Chul Soo; Kim, Mi Jung; Kim, Yang Kil; Lee, Mi Ja; Park, Ki Hoon; Noh, Tae Hwan

Research in Plant Disease 2008;14(3):153-158.
Published online December 30, 2008.

맥류의 흰가루병 저항성 검정을 위한 실내 유묘 대량검정 방법

박종철, 박철수, 김미정, 김양길, 이미자, 박기훈, 노태환

An Easy Seedling Method to Screen Resistance of Powdery Mildew of Barley and Wheat

Jong Chul Park, Chul Soo Park, Mi Jung Kim, Yang Kil Kim, Mi Ja Lee, Ki Hoon Park, Tae Hwan Noh

Abstract

This study was conducted to develop a easy seedling method to test large amounts of barley and wheat cultivars for resistance of powdery mildew. In addition, we also examined the resistance of genetic resources that have been used in barley and wheat breeding programs in Korea. This seedling test used paper bag with seeds can be completed within three weeks. 10-day seedlings were available to inoculation and 8 days was needed for result reading. This method can test at least 180 collections at one time. we can identify the two resistant types by leave symptoms showed non infection and necrotic spots. Among the 79 Korean barley cultivars, only two cultivar, ``Sangrokbori`` and ``Dajinbori`` were resistant and ``Jejubori`` showed moderate resistant. There was no resistant in hulless barley and wheat cultivars. It was same results in comparison of earlier resistant reports in field test. We confirm that this method could using in test of powdery mildew resistance in barley and wheat. Among the 1,401 genetic resources using in Korean breeding program, malting barley has more resistant collections comparing to 796 hulled and hulless barley and 273 wheat germplasms.

Key Words: Barley, Powdery mildew, Resistant, Seedling test, Wheat