Research in Plant Disease 2005;11(2):158-161.
Published online December 30, 2005.
길항세균 Stenotrophomonas maltophilia BW-13의 대량배양을 위한 최적 배양조건
박종영, 김한우, 김현주, 전옥주, 정순재, 최우봉, 이선우, 문병주
 
Cultivation Conditions for Mass Production of an Antagonistic Bacterium Stenotrophomonas maltophilia BW-13
Jong Young Park, Han Woo Kim, Hyun Ju Kim, Ok Ju Chun, Soon Je Jung, Woo Bong Choi, Seon Woo Lee, Byung Ju Moon
Abstract
Stenotrophomonas maltophilia BW-13 is a potent biocontrol agent to control crisphead lettuce bottom rot caused by Rhizoctonia solani. To define the optimum conditions for the mass production of the S. maltophilia BW-13, we have investigated optimum culture conditions and effects of various carbon sources on the bacterial growth. The optimum initial pH and temperature were determined as pH 6.0~7.0 and 35℃, respectively. For the selection of effective carbon source for the mass production, we tested the low molecular carbon sources such as sucrose, glucose, lactose, maltose, manose and the high molecular carbon source such as dough conditioner, rice bran, corn starch, sweet potato starch. As the results, the addition of dough conditioner in a basal medium (1.25% K2HPO4, 0.38% KH2PO4, 0.01% MgSO4 · 7H2O, 0.5% Yeast extract) was able to achieve higher cell density and the antifungal activity than others. Therefore, the basal medium containing 3% dough conditioner (named as dough conditioner medium) was finally selected the optimized media for the mass production of BW-13 strain.
Key Words: Antagonistic bacteria, Bottom rot, Rhizoctonia solani, Dough conditioner medium, Stenotrophomonas maltophilia BW-13


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