| 소형의 포자를 형성하는 Alternaria 균류의 분자생물학적 특징 |
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김병련, 박명수, 조혜선, 유승헌 |
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| Molecular Characterization of Small-Spored Alternaria Species |
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Byung Ryun Kim, Myung Soo Park, Hye Sun Cho, Seung Hun Yu |
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| Abstract |
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To establish taxonomic system of morphologically similar species of small spored Alternaria, phylogenetic analysis of internal transcribed spacer (ITS 1, ITS 2 and 5.8S rDNA) and mitochondrial small subunit (mtSSU) rDNA sequences and URP-PCR fingerprinting analysis from 11 species of Alternaria were performed. Phylogenetic analysis of ITS and mt SSU rDNA sequences revealed that 10 out of 11 species of the smallspored Alternaria were phylogenetically identical with a bootstrap value of 100%. A. infectoria only was phylogenetically differentiated from the other species. The results suggest that the 10 small-spored Alternaria species are very closely related evolutionally and the markers can not be used for differentiation of the smallspored Alternaria species. URP-PCR fingerprinting analysis from eleven species of small-spored Alternaria using 10 URP primers showed that it was possible to differentiate the species, although genetic similarities were found among the species. The Alternaria sp. from common pokeweed could be distinguished from other species by URP-PCR analysis, and it was considered as a new species. A. infectoria could be easily distinguished from the other 10 species by phylogenetic analysis of ITS and mt SSU rDNA sequences and the URPPCR fingerprinting analysis. |
| Key Words:
ITS, Mitochondrial small subunit, Small-spored Alternaria, URP-PCR |
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