Research in Plant Disease 2005;11(1):21-27.
Published online June 30, 2005.
고추 탄저병균 Colletotrichum acutatum의 포장 밀도 조사를 위한 반선택 배지의 확립 및 활용
강범관, 민지영, 김윤식, 박성우, , 김흥태
 
Semi-selective Medium for Monitoring Colletotrichum acutatum Causing Pepper Anthracnose in the Field
Beum Kwan Kang, Ji Young Min, Yun Sik Kim, Sung Woo Park, Nguyen Van Bach, Heung Tae Kim
Abstract
It was confirmed that anthracnose pathogen, Colletotrichum acutatum, could specifically grow on PDA amended with 100 μg/ml of ampicillin and tetracycline, and 100 μg/ml of mixture with carbendazim and diethofencarb. There was a positive correlation between the number of colony enumerated on semi-selective media and the disease severity on pepper fruits caused by C. acutatum. Using semi-selective media for C. acutatum, the number of pathogen on soil and plant debris infected by anthracnose pathogen was investigated. In plant debris, the colony number of C. acutatum was more than in soil. For the identification of colony appeared on semi-selective media, 10 isolates were selected randomly. They were identified as C. acutatum through PCR using C. acutatum-specific primer.
Key Words: Colletotrichum acutatum, Pepper anthracnose, Semi-selective media


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