Research in Plant Disease 2004;10(1):78-81.
Published online March 30, 2004.
잎절편(Leaf disk)을 이용한 오이 흰가루병((Sphaerotheca fusca)에 대한 내병성 검정법
이용환, 서종분, 최경주, 박인진, 양원모
 
Disease Resistance Test Method of Cucumber Powdery Mildew (Sphaerotheca fusca) Using A Leaf Disk Assay
Yong Hwan Lee, Jong Bun Seo, Kyong Ju Choi, In Jin Park, Won Mo Yang
Abstract
The resistance of 10 varieties of cucumber (Cucumis sativus L.) to powdery mildew, caused by Sphaerotheca fusca, was evaluated by a leaf disk assay. Leaf disks (10 mm in diameter) were removed from fully expanded leaves and then placed in petri dishes containing 0.16% water agar amended with benzimidazole. Leaf disks were inoculated by dropping a 10 μl of conidia suspension. Conidiophore formation of powdery mildew was the greatest at 25℃. The response of the host to powdery mildew, based on the inoculation onto disks of the first leaf, highly correlated with results obtained from harvesting stage of cucumber plants in greenhouse test (r = 0.99**). It is indicating that a leaf disk assay may precisely predict the response of cucumber plant to S.fusca.
Key Words: Cucumber, Disease resistance, Leaf disk assay, Powdery mildew


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