Research in Plant Disease 2002;8(4):250-253.
Published online December 30, 2002.
참다래 궤양병의 간편한 병원성 검정법 개발
고숙주, 이용환, 차광홍, 박기범, 박인진, 김영철
An Improved Method for Testing Pathogenicity of Pseudomonas syringae pv. actinidiae Causing Bacterial Canker of Kiwifruit
Sug Ju Ko, Yong Hwan Lee, Kwang Hong Cha, Ki Beum Park, In Jin Park, Young Cheol Kim
This research was conducted to develop a simple and effective method for pathogenicity assay of the causal agent of bacterial canker on kiwifruit. The developed method is a modified version of syringe-infiltration method that is used in the assay for the hypersensitive response assay. Bacterial cell suspensions in 50 mM potassium phosphate buffer(pH 7.5) were infiltrated using a plastic syringe with 25G needle into primary leaves of five-year-old kiwifruit. Typical symptoms of bacterial canker were observed five days after infiltration. Symptoms developed on the leaves were detected in these inocula that treated above 10(4) cfu/ml or above. Using this technique, host range of Pseudomonas syringae pv. actinidiae and three other plant pathogenic pseudomonads were investigated for 25 different plant species. The various symptoms were showed depending on different plant species and inoculated pathogen combinations. This method has the advantage that symptoms can be showed faster compared to other methods and high humid conditions are not required.
Key Words: bacterial canker, injection method, kiwifruits, pathogenicity test, Pseudomonas syringae pv. actinidiae

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