Biological control of Gray Mold Rot of Perilla Caused by Botrytis cinerea 2. Formulation of Antagonistic Bacteria and Its Control Effect

Moon, Byung Ju; Kim, Choul Soung; Song, Ju Hee; Kim, Hyun Ju; Lee, Jae Pil; Park, Hyean Cheal; Shin, Dong Bum

Research in Plant Disease 2002;8(3):184-188.
Published online September 30, 2002.

들깨 잿빛곰팡이의 생물학적 방제 2.미생물농약의 제조 및 그 방제효과

문병주, 김철승, 송주희, 김현주, 이재필, 박현철, 신동범

Biological control of Gray Mold Rot of Perilla Caused by Botrytis cinerea 2. Formulation of Antagonistic Bacteria and Its Control Effect

Byung Ju Moon, Choul Soung Kim, Ju Hee Song, Hyun Ju Kim, Jae Pil Lee, Hyean Cheal Park, Dong Bum Shin

Abstract

An antagonistic bacteria, Bacillus licheniformis N1 strain which effectively inhibited mycelial growth of gray mold rot pathogen, Botrytis cinerea was isolated from the rhizosphere of perilla crop. Powder soy formulation by B. licheniformis N1 strain as a biocontrol agent was developed for the first time and estimated its control effect on perilla leaves in this study. First of all, for the mass production of antifungal metabolites of B. licheniformis N1 strain in flask liquid culture, the most effective carbon and nitrogen source were selected as glucose and tryptone, respectively. For the formulation, vegetative biomass of B. licheniformis N1 strain from 5-day-old liquid culture in nutrient broth added glucose and tryptone was mixed with soy flour, rice flour, glucose, FeSO4 · 7H2O, and MnCl2 · 4H2O, and dried and pulverized. In plastic house test, powder soy formulation effectually controlled gray mold rot as the control value of 93.1%, was more effective than chemical fungicide, benomyl showing the control value of 86.1%. Thus, development of powder soy formulation of B. licheniformis N1 will aid large-scale application of biological control in field trials.

Key Words: Bacillus Licheniformis N1, Botrytis cinerea, gray mold rot, powder soy formulation, biological control, perilla