Detection of TMV, ToMV, and CMV from Tomato Seeds and Plants

Park, Kyung Hoon; Cha, Byeong Jin

Research in Plant Disease 2002;8(2):101-106.
Published online June 30, 2002.

토마토 종자와 식물체로부터의 TMV, ToMV 및 CMV 검출

박경훈, 차병진

Detection of TMV, ToMV, and CMV from Tomato Seeds and Plants

Kyung Hoon Park, Byeong Jin Cha

Abstract

For the detection of Tobacco masaic virus (TMV), Tomato mosaic virus (ToMV), and Cucumber mosaic virus (CMV), tomato seeds of 11 table tomato and 7 cherry tomato cultivars were assayed by DAS-ELISA. Among the cultivars, TMV and ToMV were detected from 9 cultivars at the rates lower than 20% and 16%, respectively. In the assay on seed transmission rates, ToMV and CMV were detected as high as 24% and 8%, respectively, but TMV was not detected. In field survey on these viruses from tomato plants of 10 different places in Chungbuk province, ToMV and CMV were detected from most fields. TMV was detected from only 3 fields. The highest detection rates of these viruses were recorded in Cheongwon for TMV, Chungju for ToMV, and the other locality of Chungju for CMV. It was difficult to find any relationship between the growth stage of tomato and infection rates. TMV usually caused mosaic on leaves while ToMV caused various symptoms including yellows, necrosis, and mottling. CMV-infected tomato plants showed symptoms of shoestring, fern leaf, and yellows.

Key Words: CMV, contamination, tomato-seeds, TMV, ToMV