| Uncovering Candidate Pathogenicity Genes in Erwinia pyrifoliae YKB12327 via Tn5-insertion Mutagenesis |
Hualin Nie1, Mi-Hyun Lee2, Sanghee Lee1,3, Seo-Rin Ko1,4, Young-Soo Hong4,5, Jae Sun Moon1, Jun Myoung Yu6, Ah-Young Shin1,4, Suk-Yoon Kwon1,3 
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1Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea
2Crop Protection Division, National Institute of Agricultural Science, Wanju 55365, Korea
3KRIBB School of Biotechnology, Korea University of Science and Technology (UST), Daejeon 34113, Korea
4KRIBB School of Bioscience, Korea University of Science and Technology (UST), Daejeon 34113, Korea
5Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Korea
6Department of Applied Biology, Chungnam National University, Daejeon 34134, Korea |
Correspondence:
Jun Myoung Yu, Tel: +82-42-821-5768, Fax: +82-42-823-8679,
Email: junyu@cnu.ac.kr
Ah-Young Shin, Tel: +82-42-860-4341, Fax: +82-42-860-4692,
Email: shinay@kribb.re.kr
Suk-Yoon Kwon, Tel: +82-42-860-4005, Fax: +82-42-860-4692,
Email: sykwon@kribb.re.kr
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Received: May 29, 2024 Revised: July 23, 2024 Accepted: July 26, 2024 |
| Abstract |
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Erwinia pyrifoliae is a gram-negative bacterial pathogen that commonly causes black shoot blight in pear and apple tree. Although the pathogenicity of this bacterial species is very similar to E. amylovora, there is no specific explanation of its pathogenic genes and mechanisms. In this study, our investigation into E. pyrifoliae pathogenicity involved generating seven YKB12327 mutant strains using Tn5 transposon mutagenesis. Observations revealed weakened growth rate and loss of pathogenicity in these mutants. Whole-genome sequencing and alignment analysis identified transposon insertions within the coding sequences of five strains and in the intergenic region of two strains. Annotation analysis elucidated genes directly or indirectly associated with pathogenicity. Notably, mutant strain MT16 displayed a transposon insertion mutation in the cyclic-di-GMP phosphodiesterase (pdeF) gene, a key player in bacterial signaling, governing microbial behavior and adaptation to environmental changes. Our findings provide insights into the genetic regulation of E. pyrifoliae pathogenicity, suggesting potential avenues for further research aimed at understanding and controlling this bacterial pathogen by targeting pdeF to mitigate apple black shoot blight disease. |
| Key Words:
Black shoot blight, Erwinia pyrifoliae, Mutagenesis, Pathogenicity, Tn-5 transposon |
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