Biological Control of Sclerotinia Rot Caused by <i>Sclerotinia sclerotiorum</i> in Lettuce Using Bacillus amyloliquefaciens HY

Son, Mi-Ra; Kim, Jin-Won

doi:2024.30.3.247

Res. Plant Dis > Volume 30(3); 2024 > Article

Research Article

Research in Plant Disease 2024;30(3):247-255.
DOI: https://doi.org/10.5423/RPD.2024.30.3.247 Published online September 30, 2024.

Bacillus amyloliquefaciens HY를 이용한 상추 균핵병의 생물학적 방제

손미라, 김진원

서울시립대학교환경원예학과

Biological Control of Sclerotinia Rot Caused by Sclerotinia sclerotiorum in Lettuce Using Bacillus amyloliquefaciens HY

Mi-Ra Son, Jin-Won Kim

Department of Environmental Horticulture, University of Seoul, Seoul 02504, Korea

Correspondence: Jin-Won Kim, Tel: +82-2-6490-2690, Fax: +82-2-6490-2684,
Email: jwkim@uos.ac.kr

Received: April 26, 2024 Revised: July 03, 2024 Accepted: July 04, 2024

Abstract

To test the antagonistic ability of Bacillus amyloliquefaciens HY against lettuce sclerotinia rot caused by Sclerotinia sclerotiorum, an in vitro test, greenhouse test and growth chamber test were conducted. As a result of in vitro dual culture test for sclerotia of B. amyloliquefaciens HY, the mycelial growth inhibition rate was 70.7%. Mycelial tip was swollen, black, and had an irregular and deformed shape. In I-plate test, the antagonistic volatile compounds produced by B. amyloliquefaciens HY, the mycelial growth inhibition rate was 57.7% and the sclerotia germination inhibition rate was 46.7%. In addition, B. amyloliquefaciens HY had sideropore production capacity, phosphate solubillibty, and protease production capability, but B. amyloliquefaciens HY did not produce chitinase. In growth chamber test, the lettuce survival rate was 0% in the control group treated with only the pathogen, and 80% in the treatment group treated with of S. sclerotiorum and B. amyloliquefaciens HY by diluting 10 times of the lettuce survival rate. In addition, the lettuce survival rate of 80% was shown in the treatment group treated with the culture medium of B. amyloliquefaciens HY 50 times 1 week later after inoculation with S. sclerotiorum.

Key Words: Bacillus amyloliquefaciens HY, Biological control, Lettuce, Sclerotinia rot, Sclerotinia sclerotiorum