| 벼 깨씨무늬병 Bipolaris oryzae의 포자 형성 방법 개선 |
장설화1, 김서연1,2, 김신화3, 정현정3, 박숙영1,2 
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1순천대학교식물의학과
2순천대학교 IT-Bio 융합시스템(BK21 plus) 협동과정
3국립식량과학원작물기초기반과 |
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| Optimization of Conditions for Conidial Production in Bipolaris oryzae Isolated from Rice |
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Seol-Hwa Jang1, Seyeon Kim1,2, Shinhwa Kim3, Hyunjung Chung3, Sook-Young Park1,2
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1Department of Plant Medicine, Sunchon National University, Suncheon 57922, Korea
2Interdisciplinary Program in IT-Bio Convergence System (BK21 plus), Sunchon National University, Suncheon 57922, Korea
3Crop Foundation Research Division, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Korea |
Correspondence:
Hyunjung Chung, Tel: +82-63-238-5343, Fax: +82-63-238-5191,
Email: chunghyunjung@korea.kr
Sook-Young Park, Tel: +82-61-750-5187, Fax: +82-61-750-5187,
Email: spark@scnu.ac.kr
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Received: July 27, 2024 Revised: August 19, 2024 Accepted: August 20, 2024
*Seol-Hwa Jang and Seyeon Kim contributed equally to this study as co-first authors. |
| Abstract |
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Conidial production is a critical factor in testing pathogenicity and studying the physiology and ecology of fungal pathogens. Therefore, selecting an appropriate condition and medium for consistent conidia production is essential. In this study, we investigated light conditions and suitable medium conditions using the slide culture method to establish optimal conditions for continuous spore acquisition of Bipolaris oryzae. Primarily, we observed conidial production using two B. oryzae isolates, CM23-042 and 23CM10, under two different light conditions: (1) consistent near-ultraviolet (NUV) with fluorescent light, and (2) a 12-hr shift of the NUVdark cycle. Secondly, we examined conidial formation under seven different media on potato dextrose agar (PDA), V8-Juice agar, minimal medium (MM), sucrose-proline agar (SPA), rabbit food agar (RFA), rice bran agar (RBA), and rice leaf agar (RLA). Under consistent NUV light with fluorescent conditions, conidia were induced in both isolates, whereas conidia were not produced under other conditions after 7 days post-inoculation (dpi). Moreover, B. oryzae isolate CM23-042 produced the highest number of conidia in MM, while isolate 23CM10 yielded the highest number of conidia in PDA after 7 dpi. In summary, our data demonstrated that the consistent NUV light with fluorescent conditions were most conducive for conidia induction in B. oryzae. The selection of a medium for conidiation may vary depending on the B. oryzae isolates, but using MM and PDA or SPA and RFA medium could be effective for spore induction. These findings will contribute to improving conidiation according to the characteristics of collected isolates of B. oryzae. |
| Key Words:
Bipolaris oryzae, Brown spot disease, Conidial production, Oryza sativa, Spore |
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