Res. Plant Dis > Volume 27(3); 2021 > Article
Research in Plant Disease 2021;27(3):99-106.
DOI: https://doi.org/10.5423/RPD.2021.27.3.99    Published online September 30, 2021.
Effects of Temperature and Culture Media Composition on Sporulation, Mycelial Growth, and Antifungal Activity of Isaria javanica pf185
Jang Hoon Lee1, Yong Seong Lee2, Young Cheol Kim3 
1BASF Korea Ltd., Agricultural Solutions, Seoul 04518, Korea
2Department of Research and Development, Agricultural Technology Center, Sunchang 56025, Korea
3Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea
Correspondence:  Young Cheol Kim, Tel: +82-62-530-2071, Fax: +82-62-530-0208, 
Email: yckimyc@jnu.ac.kr
Received: July 16, 2021   Revised: September 13, 2021   Accepted: September 14, 2021
Abstract
The fungal isolate Isaria javanica pf185 has potential as a mycopesticide because it demonstrates insecticidal activity against the green peach aphid and antifungal activity against Colletotrichum gloeosporioides. For commercialization of this isolate, determination of the optimal and least expensive culture conditions is required; however, these data are not currently available. This study describes the conditions for optimal development of conidia and production of metabolites for the biocontrol of the fungal pathogen. The optimal culture conditions were examined using cultures on solid agar and liquid media. High growth temperature enhanced spore formation but reduced antifungal activity in both solid and liquid media. The highest spore yield was obtained in a medium containing glucose as a carbon source and yeast extract as a nitrogen source. Soybean powder and wheat bran were effective nitrogen sources that promoted spore production and antifungal activity of the isolate. These results revealed the basic, cost-effective growth media for commercial production of a biopesticide with insecticidal and antifungal properties for use in integrated pest management.
Key Words: Antifungal activity, Growth temperature, Mycopesticide, Nitrogen sources


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