서론
재료 및 방법
수박 재배
접종원 준비
수박 덩굴쪼김병균 접종
발병 및 병 조사
Fig. 2

결과 및 고찰
접종 방법에 따른 덩굴쪼김병 발생의 차이
Table 1
Inoculation method | Inoculum concentration (conidia/ml) | Cultivar | |||
---|---|---|---|---|---|
|
|||||
Bulrojangsaeng | Soknoranggul | Seotaja | Jijonggul | ||
Root-dipping† | 1.0×106 | 0.0±0.0‡ a§ | 2.3±1.3 a | 3.5±0.7 a | 3.7±0.5 a |
1.0×107 | 0.0±0.0 a | 2.5±1.3 a | 3.8±0.6 a | 3.8±0.4 a | |
Scalpel|| | 1.0×106 | 0.0±0.0 a | 2.0±1.5 a | 3.3±1.2 a | 3.4±1.0 a |
1.0×107 | 0.0±0.0 a | 2.4±1.2 a | 3.8±0.4 a | 3.9±0.3 a | |
Tip¶ | 1.0×106 | 0.0±0.0 a | 2.2±1.3 a | 3.5±0.7 a | 3.4±1.1 a |
1.0×107 | 0.0±0.0 a | 2.1±1.4 a | 3.7±0.9 a | 3.4±1.1 a | |
Soil-drenching** | 1.0×106 | 0.1±0.3 a | 0.3±0.5 b | 0.8±1.0 b | 0.7±1.3 b |
1.0×107 | 0.0±0.0 a | 1.0±1.2 a | 1.4±1.5 b | 1.9±1.9 a |
* Ten-day-old seedlings of three watermelon (Soknoranggul, Seotaja, Jijonggul) and one watermelon-rootstock (Bulrojangsaeng) cultivars were inoculated with Fusarium oxysporum f. sp. niveum (Fon) HA. The inoculated plants were incubated in a dew chamber at 25°C for 24 h and then transferred to a growth room at 25°C with 12-h light a day. After 4 weeks, disease severity of the seedling was investigated on a scale of 0-4.
† Seedlings of each cultivar were uprooted and the roots were washed gently in water. The plant roots were inoculated with two different spore concentration (1.0×106 conidia/ml and 1.0×107 conidia/ml) of Fon HA by which the roots were dipped in spore suspensions for 30 min. The infected plants were transplanted into 40-cell plastic trays.
‡ Each value represents the mean of disease index±standard deviation of two runs with ten replicates each.
§ Values in the labeled with the same letter within columns are not significantly different in Duncan’s multiple range test at P=0.05.
|| Seedlings were inoculated with Fon HA by cutting the roots with a scalpel, and then 10 ml of spore suspension was applied to soil.
생육시기에 따른 품종간 병발생의 차이
Table 2
Cultivar | Plant growth stage | |||||||
---|---|---|---|---|---|---|---|---|
|
||||||||
7-day-old | 10-day-old | 13-day-old | 16-day-old | |||||
Bulrojangsaeng | 0.0±0.0† b‡ | R§ | 0.0±0.0 c | R | 0.0±0.0 c | R | 0.0±0.0 c | R |
Soknoranggul | 2.5±1.2 a | MR | 2.2±1.0 b | MR | 2.1±1.2 b | MR | 2.0±0.7 b | MR |
Seotaja | 3.3±0.9 a | S | 3.9±0.3 a | S | 3.5±0.8 a | S | 3.3±0.9 a | S |
Jijonggul | 3.7±0.7 a | S | 3.8±0.6 a | S | 3.9±0.3 a | S | 3.5±0.8 a | S |
* Seven-, ten-, thirteen-, sixteen-day-old seedlings of three watermelon (Soknoranggul, Seotaja, Jijonggul) and one watermelon-rootstock (Bulrojangsaeng) cultivars were inoculated with Fusarium oxysporum f. sp. niveum HA by cutting the roots with a scalpel, and then 10 ml of spore suspension (3.0×106 conidia/ml) was applied to soil. The inoculated plants were incubated in a dew chamber at 25°C for 24 h and then transferred to a growth room at 25°C with 12-h light a day. After 4 weeks, disease severity of the seedling was investigated on a scale of 0-4.
† Each value represents the mean of disease index±standard deviation of two runs with ten replicates each.
재배 온도에 따른 품종간 병발생의 차이
Table 3
Cultivar | Incubation temperature | |||
---|---|---|---|---|
|
||||
25°C | 30°C | |||
Bulrojangsaeng | 0.1±0.3† c‡ | R§ | 0.0±0.0 b | R |
Soknoranggul | 2.0±0.9 b | MR | 2.7±1.3 a | S |
Seotaja | 4.0±0.0 a | S | 3.6±1.0 a | S |
Jijonggul | 3.7±0.7 a | S | 3.5±1.0 a | S |
* Ten-day-old seedlings of three watermelon (Soknoranggul, Seotaja, Jijonggul) and one watermelon-rootstock (Bulrojangsaeng) cultivars were inoculated with Fusarium oxysporum f. sp. niveum HA by cutting the roots with a scalpel, and then 10 ml of spore suspension (3.0×106 conidia/ml) was applied to soil. The inoculated plants were incubated in dew chambers at 25°C and 30°C for 24 h and then transferred to growth rooms at 25°C and 30°C with 12-h light a day, respectively. After 4 weeks, disease severity of the seedling was investigated on a scale of 0-4.
† Each value represents the mean of disease index±standard deviation of two runs with ten replicates each.
접종원 농도에 따른 품종간 병발생의 차이
Table 4
Cultivar | Inoculum concentration (conidia/ml) | |||||||
---|---|---|---|---|---|---|---|---|
|
||||||||
3.7 × 105 | 1.1 × 106 | 3.3 × 106 | 1.0 × 107 | |||||
Bulrojangsaeng | 0.0±0.0† c‡ | R§ | 0.0±0.0 c | R | 0.0±0.0 c | R | 0.0±0.0 c | R |
Soknoranggul | 1.5±0.8 b | MR | 1.7±0.8 b | MR | 1.9±1.0 b | MR | 2.2±1.2 b | MR |
Seotaja | 2.8±1.1 ab | S | 3.7±0.5 a | S | 3.6±0.7 a | S | 3.7±0.7 a | S |
Jijonggul | 3.1±1.0 a | S | 3.7±0.7 a | S | 3.7±0.9 a | S | 4.0±0.0 a | S |
* Ten-day-old seedlings of three watermelon (Soknoranggul, Seotaja, Jijonggul) and one watermelon-rootstock (Bulrojangsaeng) cultivars were inoculated with Fusarium oxysporum f. sp. niveum HA by cutting the roots with a scalpel, and then 10 ml of each spore suspension was applied to soil. The inoculated plants were incubated in a dew chamber at 25°C for 24 h and then transferred to a growth room at 25°C with 12-h light a day. After 4 weeks, disease severity of the seedling was investigated on a scale of 0-4.
† Each value represents the mean of disease index±standard deviation of two runs with ten replicates each.
Scalpel 접종법에 의한 시판 품종들의 저항성
Table 5
Cultivar | Inoculation method | |||
---|---|---|---|---|
|
||||
Scalpel† | Root-dipping‡ | |||
Bulrojangsaeng (rootstock) | 0.0±0.0§ | R|| | 0.0±0.0 | R |
ShinFR-bulsajo (rootstock) | 0.0±0.0 | R | 0.0±0.0 | R |
Soknoranggul | 2.0±1.2 | MR | 2.5±1.3 | MR |
Seolgang102 | 3.5±1.1 | S | 3.9±0.3 | S |
Hwanggeumggul | 3.3±1.2 | S | 3.8±0.4 | S |
Bestggul | 3.6±0.7 | S | 3.5±0.5 | S |
Noranbusibok | 3.3±1.1 | S | 3.7±0.7 | S |
Jangchunggul | 4.0±0.0 | S | 3.8±0.4 | S |
Super grand prix | 3.8±0.6 | S | 3.5±0.8 | S |
Jijonggul | 4.0±0.0 | S | 3.5±0.8 | S |
Chilbokggul | 3.8±0.6 | S | 3.8±0.4 | S |
Supergold | 3.5±0.8 | S | 3.4±0.8 | S |
Busibok | 4.0±0.0 | S | 4.0±0.0 | S |
Seotaja | 3.5±1.1 | S | 3.6±0.5 | S |
Noranbok | 3.6±0.7 | S | 3.6±0.5 | S |
Wellbing | 3.9±0.3 | S | 3.5±0.5 | S |
Kokoma | 3.9±0.3 | S | 3.5±0.5 | S |
Nakdonggul | 3.7±0.9 | S | 3.9±0.3 | S |
Wonderfulggul | 3.8±0.4 | S | 3.9±0.3 | S |
Bravoggul | 3.6±0.8 | S | 4.0±0.0 | S |
Heukho | 3.7±0.9 | S | 3.8±0.4 | S |
Kamchunggul | 4.0±0.0 | S | 3.8±0.4 | S |
Nunettineggul | 4.0±0.0 | S | 3.9±0.3 | S |
* Ten-day-old seedlings of each cultivar were inoculated with Fon HA. The inoculated plants were incubated in a dew chamber at 25°C for 24 h and then transferred to a growth room at 25°C with 12-h light a day. After 4 weeks, disease severity of the seedling was investigated on a scale of 0-4.
† Seedlings of each cultivar were inoculated with Fon HA by cutting the roots with a scalpel, and then 10 ml of spore suspension (3.0×106 conidia/ml) was applied to soil.
‡ Seedlings of each cultivar were uprooted, and the roots were washed gently in water. The plants were inoculated by dipping the roots in the spore suspension (3.0×106 conidia/ml) of Fon HA for 30 min and then transplanted into 40-cell plastic trays.