초산과 젖산 혼합액에 의한 수출용 심비디움 검역선충 Bursaphelenchus xylophilus의 방제 효과 |
서윤희1 · 박지영1 · 조명래2 · 전재용3 · 김영호1* |
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Effect of Acetic and Lactic Acid Mixtures on Control of Quarantine Nematode, Bursaphelenchus xylophilus, in Exporting Cymbidium |
Yunhee Seo1, Jiyeong Park1, Myoung Rae Cho2, Jae Yong Chun3 and Young Ho Kim1* |
1Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea 2National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon 441-440, Korea 3Plant Quarantine Technology Center, Animal and Plant Quarantine Agency, Suwon 443-400, Korea |
Correspondence:
<p>Young Ho Kim</p><p>Tel : +82-, Young Ho Kim Tel : +82-2-880-4675 Fax: +82-2-873-2317 E-mail: yhokim@snu.ac.kr |
Received: July 15, 2014 Revised: September 16, 2014 Accepted: September 17, 2014 |
Abstract |
The mixture (MX) of acetic acid (AA) and lactic acid (LA) was examined for its effectiveness in the control of the pine wood nematode Bursaphelenchus xylophilus contaminated in cymbidium culture medium. Nematode mortality in vitro was nearly 100% in AA and MX at the concentrations of 5.0-1.0% (pH 2.6 ? 4.2) and in LA only at 5.0% (pH 3.5), but lowered at concentrations of 0.5-0.1% (pH 5.1-6.9) more significantly in LA than AA and MX. MX of most concentrations caused higher nematode mortality than the average response to AA and LA. All treatments of MX (0.5% and 0.25%), fosthiazate (standard and double concentrations) and culture dilution of Paenibacillus polymyxa GBR-1 (107colony-forming units/ml) reduced significantly the nematode populations in the cymbidium culture medium, compared to non-treatment control, with no significant difference among the treatments. No phytotoxicity occurred in all treatments. pH of the medium with the time after treatment and growths of 2-year-old cymbidium were not significantly different among treatments. Considering the safety and price of the organic acids, use of MX in the processes for culturing cymbidium may be a practically reliable and eco-friendly way in the control of the quarantine nematode in cymbidium. |
Key Words:
Bursaphelenchus xylophilus, Control, Cymbidium, Nematode mortality, Organic acid mixture |
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