오이 뿌리혹선충병에 대한 효율적인 저항성 검정법 확립 |
황성민 · 장경수 · 최용호 · 김진철 · 최경자* |
한국화학연구원 바이오화학연구센터 |
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Development of Efficient Screening Methods for Resistant Cucumber Plants to Meloidogyne incognita |
Sung Min Hwang, Kyoung Soo Jang, Yong Ho Choi, Jin-Cheol Kim and Gyung Ja Choi* |
Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology, Daejeon 305-600, Korea |
Correspondence:
<p>Gyung Ja Choi</p><p>Tel : +82, Gyung Ja Choi Tel : +82-42-860-7434 Fax: +82-42-861-4913 E-mail: kjchoi@krict.re.kr |
Received: February 04, 2014 Revised: May 09, 2014 Accepted: May 14, 2014 |
Abstract |
Root-knot nematodes represent a significant problem in cucumber, causing reduction in yield and quality. To develop screening methods for resistance of cucumber to root-knot nematode Meloidogyne incognita, development of root-knot nematode of four cucumber cultivars (‘Dragonsamchuk’, ‘Asiastrike’, ‘Nebakja’ and ‘Hanelbakdadaki’) according to several conditions such as inoculum concentration, plant growth stage and transplanting period was investigated by the number of galls and egg masses produced in each seedling 45 days after inoculation. There was no difference in galls and egg masses according to the tested condition except for inoculum concentration. Reproduction of the nematode on all the tested cultivars according to inoculum concentration increased in a dose-dependent manner. On the basis of the result, the optimum conditions for root-knot development on the cultivars is to transplant period of 1 week, inoculum concentration of 5,000 eggs/plant and plant growth stage of 3-week-old in a greenhouse (25 ± 5°C). In addition, under optimum conditions, resistance of 45 commercial cucumber cultivars was evaluated. One rootstock cultivar, Union was moderately resistant to the root-knot nematode. However, no significant difference was in the resistance of the others cultivar. According to the result, we suggest an efficient screening method for new resistant cucumber to the root-knot nematode, M. incognita. |
Key Words:
Cucumis sativus , Cultivar, Host resistance, Meloidogyne incognita |
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