| 키틴분해세균, 키틴 및 그들의 산물이 함유된 미생물제에 의한 오이의 뿌리혹선충(Meloidogyne spp.) 방제 |
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하우종1 · 김영철1 · 정현채2 · 박서기3* |
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1전남대학교 식물생명공학부, 2순천대학교 산림자원학과, 3순천대학교 식물의학과 |
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| Control of the Root-Knot Nematode (Meloidogyne spp.) on Cucumber by a Liquid Bio-Formulation Containing Chitinolytic Bacteria, Chitin and Their Products |
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Woo Jong Ha1, Young Cheol Kim1, Hyunchae Jung2 and Seur Kee Park3* |
1Department of Plant Biotechnology and Biotechnology Research Institute, Chonnam National University, Gwangju 500-757, Korea
2Department of Forest Resources, Sunchon National University, Sunchon 540-742, Korea
3Department of Agricultural Biology, Sunchon National University, Sunchon 540-742, Korea |
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Correspondence:
<p>Seur Kee Park</p><p>Tel : +82, Seur Kee Park Tel : +82-61-750-3864
Fax: +82-61-750-3268
E-mail: parksk@sunchon.ac.kr |
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Received: March 31, 2014 Revised: March 23, 2014 Accepted: May 27, 2014 |
| Abstract |
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A liquid bio-formulation containing chitinolytic bacteria, chitin and their products was assessed for its potential biological control against root-knot nematodes on cucumber. The bio-formulation was prepared by cultures of three chitinolytic bacteria, Chromobacterium sp. strain C-61, Lysobacter engymogenes and Serratia plymuthica in minimal medium supplemented with chitin. Under pot conditions, the bio-formulation showed better growth of cucumber plants, and less root galls and population density of Meloidogyne spp. than control media without the bio-formulation. In a greenhouse, 75-fold diluted bio-formulations were treated instead of water around cucumber plants through hoses for drip irrigation six times at 5-day intervals from the transplanting date. After 30 and 60 days, the treatment provided about 7% and 10% enhancement in the plant height and about 78% and 69% reduction in population density of Meloidogyne spp. in the rhizosphere, respectively. In addition, the experiments showed that the control effects occurred only in the soils contacted with the bio-formulation. Undiluted bio-formulations were drenched three times at 10-day intervals around cucumber plants severely infested with Meloidogyne spp. The treatment showed about 37% plant enhancement without dead plants compared with 37% death in the untreated control, and about 82% nematode reduction. These results suggest that the bio-formulation can be practically used to control the root-knot nematode on cucumber. |
| Key Words:
Bio-formulation, Chitinolytic bacteria, Cucumber, Root-knot nematode |
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