Research in Plant Disease 2014;20(1):37-49.
Published online December 15, 2014.
선충 억제 활성 식물 추출물의 선발
김동근1* · 류영현1 · 허창석1 · 류정아1 · 연일권1 · 이윤수2
1유기농업연구소, 2강원대학교 식물자원응용공학과
 
Screening of Plant Extracts for Nemastatic Activity
Donggeun Kim1*, Younghyun Ryu1, Changseok Huh1, Junga Ryu1, Ilkwon Yeon1 and Younsu Lee2
1Organic Agriculture Research Institute, Uiseong 769-803, Korea
2Department of Applied Plant Science, Kangwon National University, Chuncheon 200-701, Korea
Correspondence:  <p>Donggeun Kim</p><p>Tel : +82-,

Donggeun Kim

Tel : +82-54-832-9669
Fax: +82-54-833-1359
E-mail: kimdgkr@korea.kr

Received: February 11, 2014   Revised: March 12, 2014   Accepted: March 20, 2014
Abstract
A total of 2,714 methanol extracts of 1,677 plant species in 178 families were screened for nemastatic activity against Rhabditis sp. by 96-well microplate bioassay. The plant extracts with a concentration of 5,000 μg/ml were mixed with aqueous nematode solution containing about 20 Rhabditis sp. and their activity was examined daily for 7 days. Out of 2,714 plant extracts examined in this test, 2,362 (87.0%) showed no negative influence on the nematode activity, while 187 (6.9%) inhibited nematode activity about 50%, 95 (3.5%) inhibited nematode activity over 90%, and 70 (2.6%) rather enhanced nematode activity. Among those showing over 50% nemastatic activity, 25 extracts were randomly selected for further screening with Rhabditis sp. and with juveniles of Meloidogyne incognita. The screening revealed that 11 extracts (44%) were consistently nemastatic. The 282 extracts with nemastatic activity could be of use in future studies on nematicidal plants.
Key Words: Meloidogyne sp. Nematicidal, Nemastatic, Plant extracts, Rhabditis
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