Identification of Cherry green ring mottle virus on Sweet Cherry Trees in Korea

Research in Plant Disease 2013;19(4):326-330.
Published online December 30, 2013.

국내 양앵두나무에서 발생한 Cherry green ring mottle virus 동정

조인숙 ( In Sook Cho ) , 최국선 ( Gug Seoun Choi ) , 최승국 ( Seung Kook Choi )

Identification of Cherry green ring mottle virus on Sweet Cherry Trees in Korea

In-Sook Cho*, Gug-Seoun Choi and Seung-Kook Choi

Horticultural and Herbal Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon 441-440, Korea

Received: October 11, 2013 Revised: November 05, 2013 Accepted: November 05, 2013

Abstract

During the 2012 growing season, 154 leaf samples were collected from sweet cherry trees in Hwaseong, Pyeongtaek, Gyeongju, Kimcheon, Daegu, Yeongju and Eumseong and tested for the presence of Cherry green ring mottle virus (CGRMV). PCR products of the expected size (807 bp) were obtained from 6 samples. The PCR products were cloned and sequenced. The nucleotide sequences of the clones showed over 88% identities to published coat protein sequences of CGRMV isolates in the GenBank database. The sequences of CGRMV isolates, CGR-KO 1?6 shared 98.8 to 99.8% nucleotide and 99.6 to 100% amino acid similarities. Phylogenetic analysis indicated that the Korean CGRMV isolates belong to the group II of CGRMV coat protein genes. The CGRMV infected sweet cherry trees were also tested for Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV), Cherry necrotic rusty mottle virus (CNRMV), Cherry mottle leaf virus (CMLV), Cherry rasp leaf virus (CRLV), Cherry leafroll virus (CLRV), Cherry virus A (CVA), Little cherry virus 1 (LChV1), Prune dwarf virus (PDV) and Prunus necrotic ringspot virus (PNRSV) by RT-PCR. All of the tested trees were also infected with ACLSV.

Key Words: CGRMV, RT-PCR, Sequence analysis, Sweet cherry