Research in Plant Disease 2013;19(4):288-293.
Published online December 30, 2013.
열처리와 경정배양을 이용한 바이러스 무병 사과 생산 시스템
이건섭 ( Gun Sup Lee ) , 김정희 ( Jeong Hee Kim ) , 김현란 ( Hyun Ran Kim ) , 신일섭 ( Il Sheob Shin ) , 조강희 ( Kang Hee Cho ) , 김세희 ( Se Hee Kim ) , 신주희 ( Ju Hee Shin ) , 김대현 ( Dae Hyun Kim )
 
Production System of Virus-free Apple Plants Using Heat Treatment and Shoot Tip Culture
Gunsup Lee1*, Jeong Hee Kim2*, Hyun Ran Kim3, Il Sheob Shin1, Kang Hee Cho1, Se Hee Kim1, Juhee Shin1 and Dae Hyun Kim1**
1Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon 440-706, Korea
2Apple Research Station, National Institute of Horticultural and Herbal Science, Rural Development Administration, Gunwi 716-812, Korea
3Future & Creation Strategy Team, Rural Development Administration, Suwon 441-707, Korea
Received: November 12, 2013   Revised: November 21, 2013   Accepted: November 25, 2013
Abstract
In worldwide, viral diseases of apple plants has caused the serious problems like reduced production and malformation of fruits. Also, the damages of apple plants by virus and/or viroid infection (Apple chlorotic leaf spot virus, Apple stem grooving virus, Apple mosaic virus, and Apple scar skin viroid) were reported in Korea. However there is few report about the protection approach against the infection by apple viruses. Therefore, this paper introduced the experimental protocol for the development of virus-free apple cultivars (Danhong, Hongan, Saenara, Summerdream). Apple plants were treated at 37oC for 4 weeks and shoot tips were cultured in vitro. After heat treatment, the detection of apple viruses was performed by RT-PCR using virusspecific detection primers in new apple cultivars. With the heat treatments followed by in vitro shoot tip culture, the proportion of virus-free stocks of ‘Danhong’, ‘Hongan’, ‘Saenara’, and ‘Summerdream’ was 28%, 16%, 12%, and 12%, respectively. Taken together, this approach can be a good tool for production of virus-free apple stocks.
Key Words: Apple chlorotic leaf spot virus, Apple mosaic virus, Apple scar skin viroid, Apple stem grooving virus, Heat treatment
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