강원도 마늘 주산지 재배마늘의 바이러스 감염 실태 |
신지은 ( Ji Eun Shin ) , 이영규 ( Yeong Gyu Lee ) , 김병섭 ( Byung Sup Kim ) |
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Incidence of Viral Infection of Main Garlic Growing Areas of Gangwon Province in Korea |
Ji-Eun Shin1, Yeong-Gyu Lee2 and Byung-Sup Kim1* |
1Department of Plant Science, Gangneung-Wonju National University, Gangneung 210-702, Korea 2Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration, Pyeongchang 232-955, Korea |
Received: January 28, 2013 Revised: July 10, 2013 Accepted: July 10, 2013 |
Abstract |
This research was conducted to investigate the incidence of viral infection in Samcheok-shi and Jeongseongun, main garlic growing areas of Gangwon province in Korea. Four primers sets (GLV, LYSV, GCLV, OYDV) were used for RT-PCR to test of 100 samples collected from each location. Infection rates of GLV, LYSV, GCLV and OYDV of garlic samples from Samcheok-shi and Jeongseon-gun were 95, 95, 92 and 33%, respectively. All garlic samples tested in this research were infected at least one virus. Coinfection of 3 kinds of virus (GLV, LYSV and GCLV) and 4 kinds of virus (GLV, LYSV, GCLV and OYDV) were 60 and 25%, respectively. While infections of GLV, LYSV and GCLV were evenly found in both two regions, OYDV infection of garlic samples collected from Jeongseon-gun was higher than Samcheok-shi. Viral infections at garlic fields of whole country and Gangwon province are very serious now. Therefore, it is very necessary to develop technic for virus-free bulb and renew seed bulb of garlic. |
Key Words:
Garlic, RT-PCR, Virus-free bulb |
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