| 주요 오이 품종의 노균병에 대한 저항성 검정 |
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이중섭 ( Jung Sup Lee ) , 한경숙 ( Kyung Sook Han ) , 이성찬 ( Seong Chan Lee ) , 소재우 ( Jae Woo Soh ) |
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| Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties |
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Jung-Sup Lee*, Kyung-Sook Han, Seong-Chan Lee and Jae-Woo Soh |
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Horticultural & Herbal Environment Division, National institute of Horticultural and Herbal Science, Rural Development Administration, Suwon 441-440, Korea |
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Received: March 22, 2013 Revised: June 18, 2013 Accepted: June 20, 2013 |
| Abstract |
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This study was carried out for the downy mildew resistant test between 2010 and 2012. A set of 22 accessions belonging to 2 wild species and 20 varieties of the genus Cucumis, originating mainly from the Asian Vegetable Research and Development Center (AVRDC) Gene Centre, was evaluated for resistance to Pseudoperonospora cubensis, causal agent of cucumber downy mildew. The youngest fully expanded true leaves were found suitable for in vitro screening. Both leaf discs and full leaves could be kept fresh longer when applying 0.2 μg/ml of gibberellin acid (GA). The incubation temperature of 20oC was found to be the most suitable temperature for symptom development comparing with 15 and 25oC. Symptom development was faster when contact diseased leaf discs (2 weeks after inoculation) on to fresh leaf samples comparing with using conidia suspension (105 spores/ml). The numbers of spots in ‘C-19’ were lower than other varieties. ‘C-19’ variety was also showed the highest level of downy mildew resistant at 20oC chamber in 6 days after inoculating with pathogen and displayed 0.90 (under 10%) of the infected rate. However, other varieties displayed susceptible in the pathogen sprayed plots. ‘C-19’ was the most resistant variety and no lesion was observed. Based on all data, ‘C-19’ can be a useful variety for the prevention of downy mildew. |
| Key Words:
Cucumis sativus, Fruit number, Germplasm evalution, Vegetable breeding |
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