Development of Efficient Screening Method for Resistance of Cabbage Cultivars to Black Rot Disease Caused by Xanthomonas campestris pv. campestris

Research in Plant Disease 2013;19(2):95-101.
Published online June 30, 2013.

양배추 검은썩음병에 대한 효율적인 저항성 검정법

이지현 ( Ji Hyun Lee ) , 김진철 ( Jin Cheol Kim ) , 장경수 ( Kyoung Soo Jang ) , 최용호 ( Yong Ho Choi ) , 안경구 ( Kyoung Gu Ahn ) , 최경자 ( Gyung Ja Choi )

Development of Efficient Screening Method for Resistance of Cabbage Cultivars to Black Rot Disease Caused by Xanthomonas campestris pv. campestris

Ji Hyun Lee1, Jin-Cheol Kim1, Kyoung Soo Jang1, Yong Ho Choi1, Kyoung Gu Ahn2 and Gyung Ja Choi1*

¹Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology, Daejeon 305-600, Korea
²Joeun Seeds, Goesan 367-833, Korea

Received: March 25, 2013 Revised: May 28, 2013 Accepted: June 08, 2013

Abstract

Black rot caused by Xanthomonas campestris pv. campestris (Xcc) is one of the most serious diseases of crucifers world-wide. To establish the efficient screening method for resistant cabbage to Xcc, different inoculation methods, inoculation positions, growth stages of seedlings, and incubation temperatures after inoculation were investigated with the seven cabbage cultivars showing different resistance degrees to the pathogen. Clipping with mouse-tooth forceps was better inoculation method than piercing with 18 pins or cutting with scissors to distinguish the level of resistance and susceptibility. In inoculation using mouth-tooth forceps, clipping the edges of the leaves near veins is more effective than injuring the veins of the leaves directly. In addition, the inoculated plants kept at 22oC showed more clear resistant and susceptible responses than those kept at 26 or 30oC. On the basis of the results, we suggest that an efficient screening method for resistance of cabbage cultivars to black rot is to clip the edges of the leaves near veins of the four-week-old seedlings with mouth-tooth forceps dipped in a suspension of Xcc at a concentration of 7 × 10⁷ cfu/ml and incubate the inoculated plants in a growth room at 22^oC with 12-hr light a day.

Key Words: Black rot, Breeding, Crucifer crop, Disease resistance, Resistant screening