| Potato virus Y에 의한 하령 감자의 괴경 괴저증상 |
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이영규 ( Young Gyu Lee ) , 김점순 ( Jeom Soon Kim ) , 김주일 ( Ju Il Kim ) , 박영은 ( Young Eun Park ) |
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| Superficial Tuber Necrosis in Potato Cultivar ‘Haryeong’ Caused by Potato virus Y |
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Young-Gyu Lee*, Jeom-Soon Kim, Ju-Il Kim and Young-Eun Park |
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Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration, Pyeongchang 232-955, Korea |
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Received: November 07, 2011 Revised: March 22, 2013 Accepted: March 25, 2013 |
| Abstract |
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Potato cv. ‘Haryeong’ was bred with high solids, resistance to late blight and good culinary quality. It has been registered as new potato variety in 2005. Tuber necrosis symptoms such as severe superficial necrosis, raised surface lesions and ringed necrotic areas were found in tubers of ‘Haryeong’ during storage of seed potatoes in 2010. Potato virus Y (PVY) was detected from these symptomatic tubers by the analysis of RTPCR using a primer set specific to coat protein gene of PVY. The nucleotide sequence of RT-PCR product (PVYHkr) was determined and compared to those of other strains, such as PVYKor, PVYN, PVYNTN, PVYO, and PVYC registered in GeneBank. The result showed that PVYHkr was exactly the same as PVYKor, Korean isolate reported in 2005, except two nucleotides. To verify the PVY was responsible for the tuber necrosis symptoms shown in the tubers of ‘Haryeong’, a bioassay was done using two viruses (PVY and Potato leafroll virus) and five potato cultivars (‘Haryeong’, ‘Superior’, ‘Atlantic’, ‘Dejima’, and ‘Chubaek’). As expected, the same necrosis symptom appeared in tubers of ‘Haryeong’ infected with PVY only during the storage period. |
| Key Words:
Haryeong, Potato, Potato virus Y (PVY), Tuber necrosis symptom, Virus |
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