Sequence Analysis of Segments 8 and 10 of Rice black-streaked dwarf virus from Maize Plants

Research in Plant Disease 2012;18(4):387-390.
Published online December 30, 2012.

옥수수에 발생한 벼검은줄오갈병 바이러스 분절게놈 S8 및 S10 전 염기서열 분석

이봉춘 ( Bong Choon Lee ) , 조상윤 ( Sang Yun Cho ) , 윤영남 ( Young Nam Yoon ) , 강인정 ( In Jeong Kang ) , 곽도연 ( Do Yeon Kwak ) , 신동범 ( Dong Bum Shin ) , 강항원 ( Hang Won Kang )

Sequence Analysis of Segments 8 and 10 of Rice black-streaked dwarf virus from Maize Plants

Bong Choon Lee*, Sang-Yun Cho, Young-Nam Yoon1, In Jeong Kang, Do Yeon Kwak1, Dong Bum Shin and Hang-Won Kang

Crop Environment Research Division, National Institute of Crop Science, Rural Development Administration, Suwon 441-857, Korea
¹Functional Crop Resource Development Division, National Institute of Crop Science, Rural Development Administration, Miryang 627-803, Korea

Received: November 05, 2012 Revised: November 11, 2012 Accepted: November 16, 2012

Abstract

Rice black-streaked dwarf virus (RBSDV) was reported to occur on maize plants in Gochang-gun of Jeonllabuk-do region in 2011. The symptoms typically include stunted and deformed leaves. Virus infected plants usually produce poor or no head. RT-PCR analysis of genomic dsRNA extracted from the plant confirmed the infection. Specific primers for full length genome of segments 8 and 10 were used for RNA amplification. Full-length genomes of S8 and S10 were cloned and sequenced. Sequence analysis revealed that the S8 and S10 sequences of the maize isolate were same with rice isolate in size, 1,936 nt and 1,801 nt, respectively. In comparison with rice RBSDV, S8 and S10 showed 94.9?99.6% and 94.1?98.4% sequence identity, respectively. Phylogenetic analysis showed that RBSDV S8 and S10 of maize plants are categorized into the same group as RBSDV of rice plant.

Key Words: DsRNA, RBSDV, Sequence