Screening for Resistance to Fusarium oxysporum f. sp. lycopersici Race 3 Using Molecular Marker in Tomato Germplasm

Research in Plant Disease 2012;18(4):304-309.
Published online December 30, 2012.

분자마커를 이용한 토마토 시들음병 race 3 저항성 토마토 유전자원 탐색

허온숙 ( On Sook Hur ) , 노나영 ( Na Young Ro ) , 고호철 ( Ho Cheol Ko ) , 김상규 ( Sang Gyu Kim ) , 이주희 ( Ju Hee Rhee ) , 곽재균 ( Jae Gyun Gwag ) , 오세종 ( Se Jong Oh )

Screening for Resistance to Fusarium oxysporum f. sp. lycopersici Race 3 Using Molecular Marker in Tomato Germplasm

On Sook Hur*, Na Young Ro, Ho Cheol Ko, Sang Gyu Kim, Ju Hee Rhee, Jae Gyun Gwag and Se Jong Oh

National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-853, Korea

Received: October 05, 2012 Revised: December 13, 2012 Accepted: December 14, 2012

Abstract

Fusarium wilt, caused by three races of Fusarium oxysporum f. sp. lycopersici, is one of the most important disease of tomato (Solanum lycopersicum) worldwide. A total of 1,906 tomato accessions were screened for the resistance to Fusarium wilt using I-3 SNP marker and high resolution melting analysis. Results showed that 97 accessions were homozygous resistant, 8 accessions were heterozygous resistant and 1,801 were homozygous susceptible. Accessions containing resistance were identified in 65 accessions of S. lycopersicum var. lycopersicum, 13 accessions of S. lycopericum var. cerasiform, 8 accessions of S. pimpinellifolium, 3 accessions of S. habrochaites, 3 accessions of S. corneliomulleri, 1 accession of S. galapagense, 3 accessions of S. peruvianum, 1 accession of S. chilense. For accurate evaluation of the Fusarium wilt resistance, however, screening to race 1 and race 2 and bio-assay still remain to be evaluated.

Key Words: Fusarium wilt, I-3, Resistant resources