Research in Plant Disease 2012;18(4):290-297.
Published online December 30, 2012.
High Resolution Melting Analysis을 이용한 고추 유전자원의 Cucumber mosaic virus 저항성 평가
노나영 ( Na Young Ro ) , 허온숙 ( On Sook Hur ) , 고호철 ( Ho Cheol Ko ) , 김상규 ( Sang Gyu Kim ) , 이주희 ( Ju Hee Rhee ) , 곽재균 ( Jae Gyun Gwag ) , 권진경 ( Jin Kyung Kwon ) , 강병철 ( Byoung Cheorl Kang )
 
Evaluation of Resistance in Pepper Germplasm to Cucumber mosaic virus by High Resolution Melting Analysis
Na Young Ro*, On Sook Hur, Ho Cheol Ko, Sang Gyu Kim, Ju Hee Rhee, Jae-Gyun Gwag, Jin-Kyung Kwon1 and Byoung-Cheorl Kang1
National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-853, Korea
1Department of Plant Science, and Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea
Received: September 29, 2012   Revised: November 23, 2012   Accepted: December 05, 2012
Abstract
In this study, total number of 1941 Capsicum accessions conserved at RDA Genebank was evaluated for their response to Cucumber mosaic virus (CMV). These accessions were composed with 9 species originated from 89 countries, included 839 Capsicum annuum, 277 C. baccatum, 395 C. chinense, 343 C. frutescens, 49 C. pubescens, and other 38 wild pepper species (C. chacoense, C. galapagoense, etc.). Resistant to CMV was screened with the 240H02SP6 SNP marker related to the Cmr1 (Cucumber mosaic resistance 1). Eighty nine accessions of pepper germplasm were resistant to CMV based on the marker. One hundred sixty two accessions showed heterozygosity. One thousand two hundred seventy accessions were susceptible to CMV. Four hundred twenty accessions did not show distinction by 240H02SP6 marker. These 89 resistant pepper germplasm can be used in a pepper breeding program against CMV.
Key Words: Capsicum, Cucumber mosaic virus, Pepper, Resistant resources


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