Flavobacterium hercynium EPB-C313 균주를 이용한 배추 뿌리혹병 생물적 방제 |
함수상 ( Soo Sang Hahm ) , 김종태 ( Jong Tae Kim ) , 한광섭 ( Kwang Seop Han ) , 김병련 ( Byung Ryun Kim ) , 김홍규 ( Hong Kyu Kim ) , 남윤규 ( Yun Kyu Nam ) , 유승헌 ( Seung Hun Yu ) |
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Biocontrol Efficacy of Endophytic Bacteria Flavobacterium hercynim EPB-C313 for Control of Chinese Cabbage Clubroot |
Soosang Hahm*, Jongtae Kim, Kwangseop Han, Byungryun Kim, Hongkyu Kim, Yunkyu Nam and Seunghun Yu1 |
Chungnam Agricultural Research and Extension Services, Yesan, Chungnam 340-861, Korea 1Department of Applied Biology, Chungnam National University, Daejeon 305-764, Korea |
Received: June 19, 2012 Revised: August 14, 2012 Accepted: August 21, 2012 |
Abstract |
Clubroot of Chinese cabbage by Plasmodiophora brassicae, was found to be high virulent to the Chinese cabbage, turnips and cabbage. It this study, the endophytic bacteria Flavobacterium hercynium EPB-C313, which was isolated from tissues of Chinese cabbage, was investigated the antimicrobial activity on the inactivation of resting spores and its control effect on clubroot disease by bioassay. The bacterial cells, culture solutions, and culture filtrates of F. hercynium EPB-C313 inactivate the resting spores of P. brassicae with 90.4, 36.8, and 26.0%, respectively. The clubroot was inhibited with 100% by dipping the seedlings of Chinese cabbage in culture solutions of F. hercynium EPB-C313 before planting, however the chemmical ‘fluazinam’ was 91.7% in pot tests. Complex treatment were highly enhanced control efficacy with 63.7% at field of 50% diseased plants by soil incorporation with the pellet contains organic matter and F. hercynium EPB-C313, seedling drench of culture solution of F. hercynium EPB-C313 and soil drench with 10 days after planting. These results imply that the F. hercynium EPB-C313 is a very useful biological control agent of clubroot disease of Chinese cabbage. |
Key Words:
Biological control, Chinese cabbage clubroot, Flavobacterium hercynium, Endophytic bacteria |
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