Research in Plant Disease 2012;18(3):186-193.
Published online September 30, 2012.
당 분해효소를 이용한 백하수오 뿌리로부터 분리한 Pregnane Glycosides의 생전환
윤미영 ( Mi Young Yoon ) , 끄엉마이뉴엔 ( Cuong Mai Nguyen ) , 최경자 ( Gyung Ja Choi ) , 최용호 ( Yong Ho Choi ) , 장경수 ( Kyoung Soo Jang ) , 차병진 ( Byeong Jin Cha ) , 김진철 ( Jin Cheol Kim )
 
Biotransformation of Pregnane Glycosides from Cynanchum wilfordii Roots by β-Glucosidase
Mi-Young Yoon1,2, Cuong Mai Nguyen1, Gyung Ja Choi1, Yong Ho Choi1, Kyoung Soo Jang1, Byeongjin Cha2 and Jin-Cheol Kim1*
1Eco-friendly New Materials Research Group, Korea Research Institute of Chemical Technology, Daejeon 305-605, Korea
2Department of Plant Medicine, Chungbuk National University, Cheongju 361-763, Korea
Received: August 17, 2012   Revised: September 10, 2012   Accepted: September 12, 2012
Abstract
Biotransformation is an eco-friendly and efficient method for enhancing the bioavailability of biopesticide. To increase the antifungal activity of the crude extract of Cynanchum wilfordii roots against barely powdery mildew, we performed biotransformation of wilfoside C1G using β-glucosidase (cellobiase from Aspergillus niger). The mixture (G sample) of partially purified wilfoside C1G and cynauricuoside A (K1G) was treated with β-glucosidase to remove a glucopyranosyl moiety. The enzyme completely converted C1G to C1N and K1G to K1N. Optimal conditions for enzymatic biotransformation of G sample were determined to be 10% ethanol, 1,555 μU β-glucosidase/ml, pH 5, and 45oC. In in vivo experiment, the G sample transformed by β- glucosidase showed stronger antifungal activity against barley powdery mildew than the non-treated G sample. These results suggest that β-glucosidase biotransformation can be applied to increase the antifungal activity of the crude extract of C. wilfordii roots against powdery mildews.
Key Words: Barley powdery mildew, Biotransformation, Cynanchum wilfordii, β-Glucosidase, Wilfoside
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