Research in Plant Disease 2012;18(2):109-116.
Published online June 30, 2012.
보문 : Bacillus subtilis GDYA-1로부터 분리한 benzoic acid의 식물병원성 곰팡이에 대한 항균활성
윤미영 ( Mi Young Yoon ) , 서국화 ( Kook Hwa Seo ) , 이상현 ( Sang Heon Lee ) , 최경자 ( Gyung Ja Choi ) , 장경수 ( Kyoung Soo Jang ) , 최용호 ( Yong Ho Choi ) , 차병진 ( Byeong Jin Cha ) , 김진철 ( Jin Cheol Kim )
 
Antifungal Activity of Benzoic Acid from Bacillus subtilis GDYA-1 against Fungal Phytopathogens
Mi-Young Yoon1,2, Kook Hwa Seo3, Sang Heon Lee3, Gyung Ja Choi1, Kyoung Soo Jang1, Yong Ho Choi1, Byeongjin Cha2 and Jin-Cheol Kim1*
1Ecofriendly New Materials Research Group, Korea Research Institute of Chemical Technology, Daejeon 305-605, Korea
2Department of Plant Medicine, Chungbuk National University, Cheongju 361-763, Korea
3Green Dynamics Corp., Gwang-Ju 506-253, Korea
Received: May 25, 2012   Revised: June 14, 2012   Accepted: June 15, 2012
Abstract
A bacterial strain antagonistic to some fungal phytopathogens was isolated from the stem of a Persimmon tree in Yeongam, Korea. This bacterium was identified as Bacillus subtilis by 16S rRNA gene sequencing and designated as B. subtilis GDYA-1. In in vivo experiment, the fermentation broth exhibited antifungal activities against Magnaporthe oryzae on rice plants, Phytophthora infestans on tomato plants, and Puccinia recondita on wheat plants. We isolated one antifungal compound and its chemical structure was determined by mass and 1H-NMR spectral data. The antifungal substance was identified as benzoic acid. It inhibited mycelial growth of M. oryzae, Rhizoctonia solani, Sclerotinia sclerotiorum, and P. capsici with minimum inhibition concentration (MIC) values, ranging from 62.5 to 125 μg/ml. Moreover, the substance effectively suppressed Phytophthora blight of red pepper caused by P. capsici in a pot experiment. To the author’s knowledge, this is the first report on the antifungal activity of benzoic acid against phytopathogenic fungi. Benzoic acid and B. subtilis GDYA-1 may contribute to environmental-friendly protect crops from phytopathogenic fungi.
Key Words: Biological control, Biopesticides, Plant diseases


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