| 보문 : 양배추 및 브로콜리 뿌리혹병에 대한 효율적인 저항성 검정 방법 확립 |
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조수정 ( Su Jung Jo ) , 심선아 ( Sun Ah Shim ) , 장경수 ( Kyoung Soo Jang ) , 최용호 ( Yong Ho Choi ) , 김진철 ( Jin Cheol Kim ) , 최경자 ( Gyung Ja Choi ) |
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| Development of Efficient Screening Method for Resistant Cabbage and Broccoli to Plasmodiophora brassicae |
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Su-Jung Jo, Sun-Ah Shim, Kyoung Soo Jang, Yong Ho Choi, Jin-Cheol Kim and Gyung Ja Choi* |
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Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology, Daejeon 305-600, Korea |
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Received: March 02, 2012 Revised: May 21, 2012 Accepted: June 08, 2012 |
| Abstract |
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Clubroot caused by Plasmodiophora brassicae Woron. is one of the most important diseases in Brassica crops worldwide. To establish more simple and reliable screening method for resistant cabbage and broccoli to P. brassicae, the development of clubroot on the plants according to inoculum concentration and incubation period after inoculating with the pathogen was investigated using P. brassicae GN1 isolate (race 9). To facilitate and acquire precise result of resistance screening of cabbage and broccoli to clubroot, 14-day-old seedlings were inoculated by drenching roots with the spore suspension of P. brassicae to give inoculum density of 2.5× 109 spores/pot. To develop the disease, the inoculated seedlings were incubated in a growth chamber at 20oC for 3 days, and then cultivated in a greenhouse (20±5oC) for five weeks. Under the optimum conditions, 16 cabbage and 17 broccoli cultivars were tested for resistance to four field isolates (GN1, GN2, GS and YC) of P. brassicae collected from four regions in Korea. Among them, some cabbage and broccoli cultivars showed different resistance response to three isolates (GN1, GN2 and GS) determined as race 9 by using the differential varieties of Williams. On the other hand, all the tested cultivars were highly susceptible to YC isolate (race 2). The results suggest that this method is efficient screening method of cabbage and broccoli for resistance to P. brassicae. |
| Key Words:
Breeding, Clubroot, Crucifer crops, Race, Resistance |
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