Research in Plant Disease 2004;10(4):331-336.
Published online December 31, 2004.
결구상추 균핵병균(Sclerotinia sclerotioum)에 대한 길항세균의 분리 및 동정
김한우, 이광렬1, 백정우1, 김현주1, 박종영1, 이진우1, 정순재1, 문병주1,*
동아대학교 농업생명연구소, 1동아대학교 생명자원과학대학
 
Isolation and Identification of Antagonistic Bacterium Active against Sclerotinia sclerotioum Causing Sclerotinia Rot on Crisphead Lettuce
Han-Woo Kim, Kwang-Youll Lee1, Jung-Woo Baek1, Hyun-Ju Kim1, Jong-Young Park1, Jin-Woo Lee1, Soon-Je Jung1, Byung-Ju Moon1,*
Center for Biotechnology, Dong-A University, Busan 604-714, Korea
1College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea
Correspondence:  Byung-Ju Moon,Phone)+82-51-200-7554, Fax)+82-51-200-6993, E-mail)bjmoon@daunet.donga.ac.kr
Received: November 22, 2004
Abstract
The fungus genus Sclerotinia contains a number of important plant pathogens. Vegetable growers in our country are probably most familiar with Sclerotinia sclerotiorum, the causes of sclerotinia rot on crisphead lettuce. S. sclerotiorum has a wide host range which can include lettuce as well as crops such as broccoli, cabbage, carrots, celery, beans, peppers, potatoes, stocks, and tomato. Some fungicides, including benomyl, are effective in some crops, but not all. So, we isolated a antagonistic bacteria that are active on sclerotinia rot caused by S. sclerotiorum and that can be used to control it. About 702 strains had been isolated from soil around plant roots in the field. Ten strains showed strong antifungal activity against S. sclerotiorum. In pot test for antagonistic activity, A-7 strain showed high control value against the pathogen when compared with others. The strain was, therefore, selected as a biocontrol candidate against sclerotinia rot and its biochemical properties and 16S rDNA sequence was analyzed. The A-7 strain was highly related to Bacillus subtilis and B. amyloliquefaciens. To confirm precise identification, we had performed gyr A gene sequences analysis. Its sequence had 96% similarity with B. amyloliquefaciens. Consequently, the isolate was identified as B. amyloliquefaciens A-7.
Key Words: Antagonistic bacteria, Bacillus amyloliquefaciens, Biocontrol, Crisphead lettuce, Sclerotinia sclerotiorum


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