Biological Control of Phytophthora Blight and Anthracnose Disease in Red-pepper Using Bacillus subtilis S54

Lee, Gun Woong; Kim, Myung Jun; Park, Jun Sik; Chae, Jong Chan; Soh, Byoung Yul; Ju, Jae Eun; Lee, Kui Jae

Research in Plant Disease 2011;17(1):86-89.
Published online April 30, 2011.

Bacillus subtilis S54 균주를 이용한 고추 역병과 탄저병의 생물학적 방제

이건웅, 김명준, 박준식, 채종찬, 소병율, 주재은, 이귀재

Biological Control of Phytophthora Blight and Anthracnose Disease in Red-pepper Using Bacillus subtilis S54

Gun Woong Lee, Myung Jun Kim, Jun Sik Park, Jong Chan Chae, Byoung Yul Soh, Jae Eun Ju, Kui Jae Lee

Abstract

Phytophthora blight and anthracnose disease caused by Phytophthora capsici and Collectotrichum gloeosporioides are the most important devastating diseases of red pepper plants, worldwide. Five different bacterial isolates were isolated from the red pepper rhizosphere and non-rhizosphere soil and subsequently tested for antagonistic activity against P. capsisi and C. gloeosporioides. The area of the inhibition zone was taken as a measure for antagonistic activity. Among the 5 isolates tested, S54 exhibited a maximum antagonistic activity under in vitro and in vivo conditions. In greenhouse studies the isolate has successfully reduced the disease symptom. Protect value was 80.8% (Phytophthora blight) and 81.9% (Anthrancnose disease), whereas the infection rate of control plants was 21.3% and 23.2%. Based on the 16S rDNA sequence and API 50CHB Kit analysis the most effective isolate was identified as Bacillus subtilis. The results of the study indicate that the stratin S54 could be used as an potential biological control of Phytophthora blight and anthracnose disease of red pepper.

Key Words: Bacillus subtilis, Biological control, Colletotrichum gloeosporioides, Phytophthora capsici