Research in Plant Disease 2010;16(3):312-315.
Published online December 30, 2010.
무름병에 감수성인 애기장대 돌연변이체 Atstp1 선발
최창현, 김민갑, 안일평, 박상렬, 배신철, 황덕주
Selection of a Susceptible Line (Susceptible to Pectobacterium 1, Atstp1) to Soft-rot Disease in T-DNA Insertion Mutants Pool of Arabidopsis
Chang Hyun Choi, Min Gab Kim, Il Pyung Ahn, Sang Ryeol Park, Shin Chul Bae, Duk Ju Hwang
Pectobacterium carotovorum subsp. carotovorum (Pcc) causes soft rot disease in various plants. Although many studies about Pcc have been going on, little is known yet about the defense genes from plants. To identify defense associated genes in response to Pcc, we screened about 20 thousand Arabidopsis T-DNA knock out lines by inoculation with Pcc. We obtained a line (Atspt1) showing more susceptible symptom compared to WT (Col-0) on 1 day after the inoculation of Pcc on leaves of Arabidopsis with toothpicks. In this study, we optimized the system to select resistant and susceptible lines to Pcc from T-DNA inserted pool of Arabidopsis and expect the system and Atspt1 might be used for molecular breeding to produce resistant vegetables against Pcc.
Key Words: Arabidopsis, Pcc, Pectobacterium, Soft rot, T-DNA knock out Lines
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