| 마늘 푸른곰팡이병 방제용 Pantoea agglomerans 59-4의 억제기작 해석 |
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김용기, 권미경, 예완해, 홍성준, 지형진, 박종호, 한은정, 박경석, 이상엽, 이승돈 |
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| Elucidation of Mode of Action of Pantoea agglomerans 59-4 for Controlling Garlic Blue Mold |
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Yong Ki Kim, Mi Kyung Kwon, Wan Hae Yeh, Sung Jun Hong, Hyung Jin Jee, Jong Ho Park, Eun Jung Han, Kyung Seok Park, Sang Yeob Lee, Seong Don Lee |
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| Abstract |
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To screen for potential biocontrol agents against postharvest disease of garlics caused by Penicillium hirsutum, a total of 1292 isolates were isolated from the rhizoshere or rhizoplane of Allium species. Among them, S59-4 isolate was selected as a potential biocontrol agent by in vivo wounded garlic bulb assay. The isolate was identified as Pantoea agglomerans (Pa59-4) through Biolog system. Pa59-4 did not inhibit the mycelial growth of P. hirsutum in dual-culture with P. hirsutum on tryptic soy agar. In order to elucidate mode of action of Pa59-4 on biological control, nutrient competition between Pa59-4 and P. hirsutum was investigated by the simple method using tissue culture plates with cylinder inserts containing defusing membrane reported by Janisiewicz et al. (2000). The results showed that Pa59-4 effectively suppressed spore germination and mycelial growth of blue mold in the low concentration (0.5%) of garlic juice, but it did not suppress those of blue mold in the high concentration (5%) of garlic juice. This result suggests that the mechanism in biocontrol of garlic blue mold by Pa 59-4 may be involved in nutrient competition with P. hirsutum on garlic bulbs. |
| Key Words:
Garlic blue mold, Nutrient competition, Pantoea agglomerans 59-4, Postharvest disease |
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