고추역병 생물적방제 근권세균의 선발 및 근권정착 능력 연구 |
장리경, 사홍종, 왕정정, 상숙한, 신순선 |
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Selection of Biocontrol Agents against Phytophthora Blight of Pepper and Its Root Colonization Ability |
Li Jing Zhang, Hong Zhong Shi, Jing Jing Wang, Shu Xian Chang, Shun Shan Shen |
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Abstract |
Four promising biocontrol agents against Phytophthora capsici were selected from 507 bacterial isolates collected from rhizosphere soils and roots of pepper plants. In vitro experiment, these four biocontrol agents inhibited mycelial growth, germination of cystospores, and formation of zoosporangia and zoospores of Phytophthora capsici. In the pot experiment, the four biocontrol agents showed control efficiency higher than 70%. In greenhouse experiment, the isolates G28-6 gave the control value of 79.4%. These four biocontrol agents successfully colonized in the population density beyond 105 cfu/g on roots of pepper in vitro. The isolates G28-6 was identified as Pseudomonas aurantiaca, based on its cultural, morphological, and biochemical characterization and 16S rRNA gene sequence analysis. |
Key Words:
Biocontrol agent, Phytophthora blight of pepper, Root colonization |
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