표주박(Lagenaria Leucantha var. gourda)에서 분리한 서브그룹 IB계통의 Cucumber mosaic virus |
오선미, 홍진성, 류기현, 이긍표, 최장경 |
|
A Subgroup IB Isolate of Cucumber mosaic virus Isolated from Lagenaria Leucantha var. gourda |
Sun Mi Oh, Jin Sung Hong, Ki Hyun Ryu, Gung Pyo Lee, Jang Kyung Choi |
|
Abstract |
An isolate of Cucumber mosaic virus (CMV), called as Lag-CMV, was identified from Lagenaria leucantha var. gourda showing mosaic symptom, and its properties was compared to Fny-CMV (subgroup IA) and As- CMV (subgroup IB) by host reaction in several indicator plants, dsRNA analysis, RT-PCR analysis, restriction enzyme profile of the PCR products and nucleotide sequence of coat protein gene. Lag-CMV was similar to As-CMV used as a control CMV by the induced chlorotic spot on inoculated leaves and mosaic symptoms on upper leaves of N. tabacum. cv. Xanthi nc. In the cucumber and zucchini squash, Lag-CMV and As-CMV induced a mild mosaic symptoms than that of Fny-CMV. Size and shapes of local lesions on Chenophodium amaranticolor and Vigna unguiculata induced by Lag-CMV was similar those by Fny-CMV or As-CMV. In experiments of dsRNA profiles and RT-PCR analysis of coat protein gene, Lag-CMV was come within subgroup I CMV. Moreover, restriction enzyme analysis using EcoRI, SalI, MspI, XhoI, and HindIII of the RTPCR products and nucleotide sequence analysis of the coat protein gene showed that Lag-CMV belong to a member of CMV subgroup IB of the same to As-CMV. |
Key Words:
Cucumber mosaic virus, Lagenaria Leucantha var. gourda., Host reaction, Restriction enzyme profile, Subgroup IB |
|