A Subgroup IB Isolate of Cucumber mosaic virus Isolated from Lagenaria Leucantha var. gourda

Oh, Sun Mi; Hong, Jin Sung; Ryu, Ki Hyun; Lee, Gung Pyo; Choi, Jang Kyung

Research in Plant Disease 2009;15(3):254-258.
Published online December 30, 2009.

표주박(Lagenaria Leucantha var. gourda)에서 분리한 서브그룹 IB계통의 Cucumber mosaic virus

오선미, 홍진성, 류기현, 이긍표, 최장경

A Subgroup IB Isolate of Cucumber mosaic virus Isolated from Lagenaria Leucantha var. gourda

Sun Mi Oh, Jin Sung Hong, Ki Hyun Ryu, Gung Pyo Lee, Jang Kyung Choi

Abstract

An isolate of Cucumber mosaic virus (CMV), called as Lag-CMV, was identified from Lagenaria leucantha var. gourda showing mosaic symptom, and its properties was compared to Fny-CMV (subgroup IA) and As- CMV (subgroup IB) by host reaction in several indicator plants, dsRNA analysis, RT-PCR analysis, restriction enzyme profile of the PCR products and nucleotide sequence of coat protein gene. Lag-CMV was similar to As-CMV used as a control CMV by the induced chlorotic spot on inoculated leaves and mosaic symptoms on upper leaves of N. tabacum. cv. Xanthi nc. In the cucumber and zucchini squash, Lag-CMV and As-CMV induced a mild mosaic symptoms than that of Fny-CMV. Size and shapes of local lesions on Chenophodium amaranticolor and Vigna unguiculata induced by Lag-CMV was similar those by Fny-CMV or As-CMV. In experiments of dsRNA profiles and RT-PCR analysis of coat protein gene, Lag-CMV was come within subgroup I CMV. Moreover, restriction enzyme analysis using EcoRI, SalI, MspI, XhoI, and HindIII of the RTPCR products and nucleotide sequence analysis of the coat protein gene showed that Lag-CMV belong to a member of CMV subgroup IB of the same to As-CMV.

Key Words: Cucumber mosaic virus, Lagenaria Leucantha var. gourda., Host reaction, Restriction enzyme profile, Subgroup IB