Comparative Analysis of Coat Protein Gene of Isolates of Cucumber mosaic virus Isolated from Pepper Plants in Two GMO Environmental Risk Assessment Fields

Hong, Jin Sung; Park, Ho Seop; Ryu, Ki Hyun; Choi, Jang Kyung

Research in Plant Disease 2009;15(3):165-169.
Published online December 30, 2009.

GM 격리포장 내 고추에서 분리한 Cucumber mosaic virus 분리주들의 외피단백질 유전자 비교

홍진성, 박호섭, 류기현, 최장경

Comparative Analysis of Coat Protein Gene of Isolates of Cucumber mosaic virus Isolated from Pepper Plants in Two GMO Environmental Risk Assessment Fields

Jin Sung Hong, Ho Seop Park, Ki Hyun Ryu, Jang Kyung Choi

Abstract

Twelve Cucumber mosaic virus (CMV) isolates were isolated from genetically modified (GM) and non-GM Capsicum annuum in two GM fields, Namyangju and Anseong, and their properties were investigated in this study. Coat protein (CP) gene of the CMV isolates were synthesized by RT-PCR using genus-specific primers which designed to amplify a DNA fragment of 950 bp. Purified cDNA fragments were cloned into the pGEMT easy vector for sequence determination. Nucleotide sequences (internal 657 bp) of CMV isolates were compared with Fny-CMV CP sequences and there were no significant collection site specific sequence similarities found. When predicted amino acid sequences (219 amino acids) were compared with Fny-CMV CP amino acids sequences, there were 96.8% to 97.3% similarities found from Namyangju collections and 95.9% to 96.8% similarities from Anseong collections. The phylogenetic analysis with nucleotide sequences showed definite differences in CMVs which have been isolated from the two regions.

Key Words: Coat protein gene, Cucumber mosaic virus, Genetically modified pepper, Nucleotide sequence, Regional difference