Identification and Biological Activity of Two New Phytotoxins Isolated from Botrytis cinerea

Kim, Geum Jung; Yoon, Mi Young; Kim, Heung Tae; Choi, Gyung Ja; Jang, Kyoung Soo; Choi, Yong Ho; Park, Myung Soo; Cha, Byeong Jin; Kim, Jin Cheol

Research in Plant Disease 2009;15(2):112-119.
Published online August 30, 2009.

Botrytis cinerea로부터 분리한 두 개의 새로운 phytotoxin의 구조 결정 및 생물활성

김금정, 윤미영, 김흥태, 최경자, 장경수, 최용호, 박명수, 차병진, 김진철

Identification and Biological Activity of Two New Phytotoxins Isolated from Botrytis cinerea

Geum Jung Kim, Mi Young Yoon, Heung Tae Kim, Gyung Ja Choi, Kyoung Soo Jang, Yong Ho Choi, Myung Soo Park, Byeong Jin Cha, Jin Cheol Kim

Abstract

We discovered two novel phytotoxins produced by the pathogenic fungus, Botrytis cinerea. Among the twentyfive B. cinerea isolates, which were obtained from various host plants in 1994 and 1996, twenty-two showed strong or moderate pathogenicity on five plants such as cucumber, tomato, red pepper, tobacco and Chinese cabbage. The culture filtrate of the B. cinerea 2-16 strain showed the most potent phytotoxic activity in a tobacco leaf-wounding assay. Two novel phytotoxins were isolated from the liquid cultures of B. cinerea 2-16 by ethyl acetate extraction, flash silica gel column chromatography, silica gel column chromatography, Sephadex LH-20 column chromatography, preparative TLC and subsequently preparative HPLC. Their chemical structures were determined to be 3-O-acetyl botcinol and 3-O-acetyl botcinolide, respectively, by mass and NMR spectral analyses. These two phytotoxins caused leaf necrosis in a leaf-wounding bioassay, and significant electrolyte leakage from leaf tissues of tobacco. In the two bioassays tested, 3-O-acetyl botcinol exhibited stronger phytotoxic activity than 3-O-acetyl botcinolide. This is the first report on the production of both 3-O-acetyl botcinol and 3-O-acetyl botcinolide from B. cinerea.

Key Words: 3-O-acetyl botcinol, 3-O-acetyl botcinolide, Botrytis cinerea, Phytotoxin