Research in Plant Disease 2008;14(3):229-232.
Published online December 30, 2008.
과꽃에서 분리한 Cucumber mosaic virus의 성질
오선미, 김성률, 홍진성, 류기현, 이긍표, 최장경
Characterization of an Isolate of Cucumber mosaic virus Isolated from Chinese aster (Callistephus chinensis)
Sun Mi Oh, Sung Ryul Kim, Jin Sung Hong, Ki Hyun Ryu, Gung Pyo Lee, Jang Kyung Choi
An isolate of Cucumber mosaic virus (CMV), designated as Cas-CMV, was isolated from Chinese aster (Callistephus chinensis) showing severe mosaic symptom, and its properties was compared to the well-characterized Fny-CMV (subgroup IA) and As-CMV (subgroup IB) by host reaction in several indicator plants, dsRNA analysis, RT-PCR analysis, and restriction enzyme profile of the PCR products. Cas-CMV differed markedly in their host reaction to Fny-CMV or As-CMV in Cucurbita pepo cv. Black beauty. In the zucchini squash, all strains induced chlorotic spot on inoculated leaves and mosaic symptoms on upper leaves. However, symptoms induced by Cas-CMV were developed lethal necrosis on the young plants 15 to 20 days after inoculation. In experiments of dsRNA analysis and RT-PCR analysis, properties of Cas-CMV was come within subgroup I CMV. Moreover, restriction enzyme analysis using HindIII of the RT-PCR products showed that Cas-CMV belong to a member of CMV subgroup IA.
Key Words: Callistephus chinensis, Cucumber mosaic virus, Cucurbita pepo, Lethal necrosis, Subgroup IA

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