| 과꽃에서 분리한 Cucumber mosaic virus의 성질 |
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오선미, 김성률, 홍진성, 류기현, 이긍표, 최장경 |
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| Characterization of an Isolate of Cucumber mosaic virus Isolated from Chinese aster (Callistephus chinensis) |
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Sun Mi Oh, Sung Ryul Kim, Jin Sung Hong, Ki Hyun Ryu, Gung Pyo Lee, Jang Kyung Choi |
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| Abstract |
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An isolate of Cucumber mosaic virus (CMV), designated as Cas-CMV, was isolated from Chinese aster (Callistephus chinensis) showing severe mosaic symptom, and its properties was compared to the well-characterized Fny-CMV (subgroup IA) and As-CMV (subgroup IB) by host reaction in several indicator plants, dsRNA analysis, RT-PCR analysis, and restriction enzyme profile of the PCR products. Cas-CMV differed markedly in their host reaction to Fny-CMV or As-CMV in Cucurbita pepo cv. Black beauty. In the zucchini squash, all strains induced chlorotic spot on inoculated leaves and mosaic symptoms on upper leaves. However, symptoms induced by Cas-CMV were developed lethal necrosis on the young plants 15 to 20 days after inoculation. In experiments of dsRNA analysis and RT-PCR analysis, properties of Cas-CMV was come within subgroup I CMV. Moreover, restriction enzyme analysis using HindIII of the RT-PCR products showed that Cas-CMV belong to a member of CMV subgroup IA. |
| Key Words:
Callistephus chinensis, Cucumber mosaic virus, Cucurbita pepo, Lethal necrosis, Subgroup IA |
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