| Real Time PCR을 이용한 Colletotrichum acutatum과 C. gloeosporioides의 검출 |
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김승한, 권오훈 |
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| Detection of Colletotrichum acutatum and C. gloeosporioides by Real Time PCR |
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Seung Han Kim, Oh Hun Kwon |
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| Abstract |
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Real time PCR was used to discriminate Colletotrichum acutatum and C. gloeosporioides for analysis of population density. Two primers, caInt2 and cgint, used for conventional PCR to discriminate two species were modified with fluorescent dye to make probe for real time PCR. Fluorescence signals were successfully detected by fCaInt2 and vCgint probe coupled with primer pair Unicof1 and Unicor1 resulting in discrimination of C. acutatum and C. gloeosporioides by comparison of delta Rn value. |
| Key Words:
Colletotrichum acutatum, C. gloeosporioides, Detection of Colletotrichum spp, Real time PCR |
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