Research in Plant Disease 2008;14(1):71-75.
Published online April 30, 2008.
토끼풀에서 분리한 Peanut stunt virus의 성질
정구호, 전용운, 최장경, 홍진성, 류기현, 이상용
 
Some Properties of an Isolate of Peanut stunt virus Isolated from White Clover (Trifolium repens L.)
Goo Ho Jung, Yong Woon Jeon, Jang Kyung Choi, Jin Sung Hong, Ki Hyun Ryu, Sang Yong Lee
Abstract
An isolate of Peanut stunt virus (PSV), named as Tr-PSV, was isolated from white clover (Trifolium repens L) showing mosaic symptom. Tr-PSV systemically infected all plants tested in the Nicotiana spp. and induced local lesions on inoculated leaves of Chenopodium amaranticolor. However, Tr-PSV induced typical mosaic symptoms as ER-PSV on Vigna unguiculata 5 to 6 days after inoculation, while Fny-CMV used as a control virus of Cucumovirus produced local lesions on inoculated leaves. In dsRNA analysis, Tr-PSV consisted of four dsRNAs, but satellite RNA was not detected. The cDNA of coat protein gene of Tr-PSV was amplified by RT-PCR using a Cucumovirus-specific single pair primers that designed to amplify a DNA fragment of approximately 950 bp. By restriction mapping analysis using RFLP of the RT-PCR products and by serological properties of gel diffusion test, Tr-PSV belongs to a typical member of PSV subgroup I. This is the first report on the occurrence of PSV in white clover in Korea.
Key Words: Host reaction, Peanut stunt virus subgroup I, RFLP, White clover


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